Perforin Antibody (CB5.4) - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-97512
![Western Blot: Perforin Antibody (CB5.4)BSA Free [NBP1-97512]](https://resources.bio-techne.com/images/products/Perforin-Antibody-CB5-4-Western-Blot-NBP1-97512-img0001.jpg "Western Blot: Perforin Antibody (CB5.4)BSA Free [NBP1-97512]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Mouse
Cited:
Mouse
Applications
Validated:
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot
Cited:
Immunocytochemistry/ Immunofluorescence, Immunofluorescence, Immunohistochemistry
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG2A Clone # CB5.4
Format
BSA Free
Concentration
1.0 mg/ml
Product Specifications
Immunogen
Recombinant mouse perforin (aa 98-534).
Specificity
Recognizes mouse perforin (region aa 402-534).
Clonality
Monoclonal
Host
Rat
Isotype
IgG2A
Scientific Data Images for Perforin Antibody (CB5.4) - BSA Free
Western Blot: Perforin Antibody (CB5.4)BSA Free [NBP1-97512]
Western Blot: Perforin Antibody (CB5.4) [NBP1-97512] - Detection of perforin in the mouse T cell clone B6.1. (lane 1). Absence of perforin in the mouse fibroblast cell line NIH.3T3 (lane 2). The protein migrates as a 66 kDa species. Method: Cell extracts from the T cell clone B6.1 (2x10^6) were resolved by SDS-PAGE under reducing conditions, transferred to nitrocellulose and probed with the CB5.4 antibody at 1 ug/ml. Proteins were visualized using a peroxidase-conjugated antibody to rat IgG and a chemiluminescence detection system.Applications for Perforin Antibody (CB5.4) - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:10-1:500
Immunohistochemistry
1:10-1:500
Immunohistochemistry-Frozen
1:10-1:500
Immunohistochemistry-Paraffin
1:10-1:500
Immunoprecipitation
1:10-1:500
Western Blot
1:100-1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Perforin
Long Name
Perforin 1 (Pore Forming Protein)
Alternate Names
Cytolysin, FLH2, HPLH2, P1, PFP, PRF1
Gene Symbol
PRF1
Additional Perforin Products
Product Documents for Perforin Antibody (CB5.4) - BSA Free
Product Specific Notices for Perforin Antibody (CB5.4) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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