Pericentrin Antibody (PSH0-66)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-32736
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Human
Applications
Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # PSH0-66
Concentration
1 mg/ml
Product Specifications
Immunogen
Recombinant protein within human aa 201-600 / 3,336. (Uniprot: O95613)
Localization
Cytoplasm, cytoskeleton, microtubule organizing center, centrosome.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
378 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Pericentrin Antibody (PSH0-66)
Western Blot: Pericentrin Antibody (PSH0-66) [NBP3-32736]
Western Blot: Pericentrin Antibody (PSH0-66) [NBP3-32736] - Western blot analysis of Pericentrin on different lysates with Rabbit anti-Pericentrin antibody (NBP3-32736) at 1/1,000 dilution.Lane 1: HeLa cell lysate
Lane 2: HepG2 cell lysate
Lane 3: MCF7 cell lysate
Lane 4: K-562 cell lysate
Lysates/proteins at 30 ug/Lane.
Predicted band size: 378 kDa
Observed band size: 220 kDa (cleavage form)
Exposure time: 2 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32736) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.
Applications for Pericentrin Antibody (PSH0-66)
Application
Recommended Usage
Western Blot
1:1000
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS (pH7.4), 0.1% BSA and 40% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Pericentrin
Alternate Names
Kendrin, KIAA0402, MOPD2, PCN, PCNT2pericentrin B, PCNTB, pericentrin, pericentrin 2 (kendrin), pericentrin, kendrin (KIAA0402)10KENPCTN2, pericentrin-2, pericentrin-380, pericentrin-B, SCKL4kendrin
Gene Symbol
PCNT
Additional Pericentrin Products
Product Documents for Pericentrin Antibody (PSH0-66)
Product Specific Notices for Pericentrin Antibody (PSH0-66)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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