Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Cited:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all Pericentrin Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 175 and 225 of human Pericentrin/Kendrin using the numbering given in entry NP_006022.3 (GeneID 5116).

Marker

Centrosome Marker

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

378 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Pericentrin Antibody

Immunohistochemistry: Pericentrin Antibody [NB100-61071]

Immunohistochemistry: Pericentrin Antibody [NB100-61071]

Immunohistochemistry: Pericentrin Antibody [NB100-61071] - Immunofluorescent analysis of cilia in renal tissues. Sections (4 um) of renal parenchymal tissues and tumor tissues were stained with DAPI, acetylated-alpha-tubulin (Ac-tub) and pericentrin (PCNT) to mark cell nuclei and cilia. Presented images are maximal projections of confocal images of typical parenchymal tissue and a representative ccRCC. Scale bars 20 um. Statistics were determined by performing a paired t-test at a 95% confidence interval. Image collected and cropped by CiteAb from the following publication (https://ciliajournal.biomedcentral.com/articles/10.1186/2046-2530-2-2) licensed under a CC-BY license.
Western Blot: Pericentrin Antibody [NB100-61071]

Western Blot: Pericentrin Antibody [NB100-61071]

Western Blot: Pericentrin Antibody [NB100-61071] - Whole cell lysate (10 ug) from HEK293T, HeLa, Hep-G2, and K-562 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Pericentrin-Kendrinantibody used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 30 second.
Immunocytochemistry/ Immunofluorescence: Pericentrin Antibody [NB100-61071]

Immunocytochemistry/ Immunofluorescence: Pericentrin Antibody [NB100-61071]

Immunocytochemistry/Immunofluorescence: Pericentrin Antibody [NB100-61071] - Formaldehyde-fixed asynchronous HeLa cells. Antibody: Affinity purified rabbit anti-Pericentrin/Kendrin used at a dilution of 1:1,000 (1ug/ml). Detection: Red-fluorescent goat anti-rabbit IgG-heavy and light chain cross-adsorbed Antibody DyLight (R) 594 Conjugated used at a dilution of 1:100. Counterstain. DAPI (blue) and Phalloidin (green)
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Applications for Pericentrin Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:1000 - 1:5000

Immunohistochemistry-Paraffin

1:10 - 1:500

Immunoprecipitation

2 - 10 ug/mg lysate

Western Blot

1:2000 - 1:10000
Application Notes
IHC-P was reported in scientific literature (PMID: 23369289). ICC/IF reactivity reported in scientific literature (PMID: 24316073). For ICC/IF, formaldehyde or acetone fixation is recommended. Permeabilization with Triton X-100 is recommended for formaldehyde-fixed cells.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 1 using NB100-61071 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Pericentrin

Pericentrin (PCNT) is a large centrosomal protein that binds calmodulin and mediates assembly of the mitotic spindle apparatus. Depletion of PCNT has been shown to cause apoptosis and activation of the G2/M checkpoint. Recently, mutations in the pericentrin gene have been identified as the cause of primordial dwarfism type II (MOPDII) and Seckel syndrome. Additionally, in cells of Seckel syndrome patients, PCNT has been found to be important to the DNA damage response and ATR-dependent checkpoint signaling.

Alternate Names

Kendrin, KIAA0402, MOPD2, PCN, PCNT2pericentrin B, PCNTB, pericentrin, pericentrin 2 (kendrin), pericentrin, kendrin (KIAA0402)10KENPCTN2, pericentrin-2, pericentrin-380, pericentrin-B, SCKL4kendrin

Entrez Gene IDs

5116 (Human)

Gene Symbol

PCNT

UniProt

O95613 (Human)

Additional Pericentrin Products

Product Documents for Pericentrin Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Pericentrin Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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