Perilipin-2/ADFP Antibody (ADFP/1365) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-54369

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Human

Applications

CyTOF-ready, ELISA, Flow Cytometry, Protein Array, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # ADFP/1365

Format

Azide and BSA Free

Concentration

1 mg/ml

View all Perilipin-2 Antibodies »

Product Summary for Perilipin-2/ADFP Antibody (ADFP/1365) - Azide and BSA Free

Immunogen

Recombinant fragment (around aa 249-376) of human Perilipin-2/ADFP protein (exact sequence is proprietary) (Uniprot: Q99541)

Localization

Cytoplasmic

Marker

Marker of Lipid Accumulation

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Theoretical MW

48 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-53401).

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for Perilipin-2/ADFP Antibody (ADFP/1365) - Azide and BSA Free

Western Blot: Perilipin-2/ADFP Antibody (ADFP/1365)Azide and BSA Free [NBP2-54369]

Western Blot: Perilipin-2/ADFP Antibody (ADFP/1365)Azide and BSA Free [NBP2-54369]

Western Blot: Perilipin-2/ADFP Antibody (ADFP/1365) - Azide and BSA Free [NBP2-54369] - Perilipin-2/ADFP Antibody (ADFP/1365) [NBP2-54369] - recombinant Adipophilin and Jurkat Cell Lysate using Adipophilin Monoclonal Antibody (ADFP/1365).
Flow Cytometry: Perilipin-2/ADFP Antibody (ADFP/1365) - Azide and BSA Free [NBP2-54369]

Flow Cytometry: Perilipin-2/ADFP Antibody (ADFP/1365) - Azide and BSA Free [NBP2-54369]

Flow Cytometry: Perilipin-2/ADFP Antibody (ADFP/1365) - Azide and BSA Free [NBP2-54369] - Flow Cytometry of human Perilipin-2/ADFP on PBMC. Green: Isotype Control; Red: Perilipin-2/ADFP Monoclonal Antibody (ADFP/1365).
Protein Array: Perilipin-2/ADFP Antibody (ADFP/1365) - Azide and BSA Free [NBP2-54369]

Protein Array: Perilipin-2/ADFP Antibody (ADFP/1365) - Azide and BSA Free [NBP2-54369]

Protein Array: Perilipin-2/ADFP Antibody (ADFP/1365) - Azide and BSA Free [NBP2-54369] - Analysis of Protein Array containing more than 19,000 full-length human proteins using Perilipin-2/ADFP Mouse Monoclonal Antibody (ADFP/1365).

Applications for Perilipin-2/ADFP Antibody (ADFP/1365) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

0.5 - 1 ug/million cells

Western Blot

0.5 - 1 ug/ml
Application Notes
ELISA: For coating, order Ab without BSA.
Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -80C. Avoid freeze-thaw cycles.

Background: Perilipin-2

Perilipin-2/Plin-2, also known as adipose differentiation-related protein (ADFP), ADRP, and adipophilin, is a member of the perilipin family of lipid droplet (LD) proteins that functions in lipid accumulation, lipid storage, and LD formation (1,2). Perilipin-2 is ubiquitously expressed and is the major LD protein of the liver (1-3). Human perilipin-2 is 437 amino acids (aa) in length with a theoretical molecular weight of 48 kDa (1,4). Human and mouse perilipin-2 share 81.8% sequence identity. One defining structural feature of perilipin-2 and other LD-related proteins is formation of amphipathic alpha helices (1,3,5). The perilipin-2 protein contains a PAT (perilipin A, ADRP, TIP47) domain, a 11-mer repeat motif, and a 4-helix bundle (1,5). The PAT domain has a role in triglyceride (TG) stabilization, the 11-mer repeat motif functions in cytoplasmic LD binding, and the 4-helix bundle facilitates membrane binding (5). Unlike LDs coated with other perilipin family members (e.g., perilipin-1 and perilipin-5), LDs coated with perilipin-2 are more permissive to lipolysis and protective of lipids and TGs (1-3). Studies have revealed that perilipin-2 knockout (KO) in mice is associated with resistance to fatty liver disease and diet-induced obesity (1). On the other hand, overexpression of perilipin-2 causes increased lipid and TG accumulation, which is a feature of many metabolic, cardiovascular, and age-related diseases (2). Additional research has found higher levels of perilipin-2 is associated with certain cancers such as colorectal cancer and renal carcinomas, and it may be a useful biomarker for detection (2). Though more research is needed, it is possible that inhibition or blocking of perilipin-2 may be a key strategy to combatting several pathologies (2).

References

1. Itabe H, Yamaguchi T, Nimura S, Sasabe N. Perilipins: a diversity of intracellular lipid droplet proteins. Lipids Health Dis. 2017;16(1):83. https://doi.org/10.1186/s12944-017-0473-y

2. Conte M, Franceschi C, Sandri M, Salvioli S. Perilipin 2 and Age-Related Metabolic Diseases: A New Perspective. Trends Endocrinol Metab. 2016;27(12):893-903. https://doi.org/10.1016/j.tem.2016.09.001

3. Sztalryd C, Brasaemle DL. The perilipin family of lipid droplet proteins: Gatekeepers of intracellular lipolysis. Biochim Biophys Acta Mol Cell Biol Lipids. 2017;1862(10 Pt B):1221-1232. https://doi.org/10.1016/j.bbalip.2017.07.009

4. Uniprot (Q99541)

5. Chong BM, Reigan P, Mayle-Combs KD, Orlicky DJ, McManaman JL. Determinants of adipophilin function in milk lipid formation and secretion. Trends Endocrinol Metab. 2011;22(6):211-217. https://10.1016/j.tem.2011.04.003

Alternate Names

ADFP, Adipophilin, Perilipin2, PLIN2

Gene Symbol

PLIN2

Additional Perilipin-2 Products

Product Documents for Perilipin-2/ADFP Antibody (ADFP/1365) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Perilipin-2/ADFP Antibody (ADFP/1365) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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