Perilipin Antibody (GT2781)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-13615

Novus Biologicals, part of Bio-Techne
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NBP3-13615
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Predicted:

Bovine (94%), Porcine (92%), Rat (92%), Sheep (94%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # GT2781

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Perilipin Antibodies »

Product Summary for Perilipin Antibody (GT2781)

Immunogen

Recombinant protein encompassing a sequence within the center region of human Perilipin. The exact sequence is proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

56 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Centrifuge briefly prior to opening.

Scientific Data Images for Perilipin Antibody (GT2781)

Western Blot: Perilipin Antibody (GT2781) [NBP3-13615]

Western Blot: Perilipin Antibody (GT2781) [NBP3-13615]

Western Blot: Perilipin Antibody (GT2781) [NBP3-13615] - Human adipose (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Perilipin antibody [GT2781] (NBP3-13615) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody, and the signal was developed with Trident femto Western HRP Substrate.
Immunohistochemistry-Paraffin: Perilipin Antibody (GT2781) [NBP3-13615]

Immunohistochemistry-Paraffin: Perilipin Antibody (GT2781) [NBP3-13615]

Immunohistochemistry-Paraffin: Perilipin Antibody (GT2781) [NBP3-13615] - Perilipin antibody [GT2781] detects Perilipin protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse white adipocyte. Perilipin stained by Perilipin antibody [GT2781] (NBP3-13615) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Western Blot: Perilipin Antibody (GT2781) [NBP3-13615]

Western Blot: Perilipin Antibody (GT2781) [NBP3-13615]

Western Blot: Perilipin Antibody (GT2781) [NBP3-13615] - Mouse tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Perilipin antibody [GT2781] (NBP3-13615) diluted at 1:5000. The HRP-conjugated anti-mouset IgG antibody (NBP2-19382) was used to detect the primary antibody.

Applications for Perilipin Antibody (GT2781)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

Assay dependent

Immunohistochemistry

Assay dependent

Immunohistochemistry-Paraffin

Assay dependent

Western Blot

1:1000-1:10000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS pH7, 20% Glycerol

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Perilipin

The perilipin family includes lipid droplet-associated protein (perilipin) and adipose differentiation-related protein (adipophilin). Perilipin is a modulator of both adipocyte lipid metabolism and adipophilinis involved in the development and maintenance of adipose tissue. Perilipin acts as a protective coating from the body's natural lipases--such as hormone-sensitive lipase--that break triglycerides into glycerol and free fatty acids for use in metabolism, a process called lipolysis. It is also an important regulator of lipid storage and its expression is elevated in obese animals and humans.

Alternate Names

PERI, PLIN, PLIN1

Gene Symbol

PLIN1

Additional Perilipin Products

Product Documents for Perilipin Antibody (GT2781)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Perilipin Antibody (GT2781)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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