Skip to main content

Perilipin Antibody [Alexa Fluor® 488]

Novus Biologicals, part of Bio-Techne | Catalog # NB110-40760AF488

Novus Biologicals, part of Bio-Techne
Catalog #
Availability
Size / Price
Qty
Loading...
NB110-40760AF488

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Porcine, Fish

Cited:

Human

Predicted:

Bovine (92%), Sheep (92%). Backed by our 100% Guarantee.

Applications

Validated:

Flow Cytometry, Imaging Mass Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

IF/IHC, Immunohistochemistry

Label

Alexa Fluor 488 (Excitation = 488 nm, Emission = 515-545 nm)

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This Perilipin Antibody was developed against a synthetic peptide made to a region between residues 450-522 (C-terminus) of the human Perilipin protein. [Swiss-Prot# O60240]

Reactivity Notes

Fish reactivity reported in scientific literature (PMID: 27012897).

Localization

Endoplasmic reticulum, Lipid droplet (surface-associated).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Perilipin Antibody [Alexa Fluor® 488]

Flow Cytometry: Perilipin Antibody [Alexa Fluor® 488] [NB110-40760AF488] - An intracellular stain was performed on U2OS cells with Perilipin Antibody NB110-40760AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.
Flow Cytometry: Perilipin Antibody [Alexa Fluor® 488] [NB110-40760AF488] - An intracellular stain was performed on MCF7 cells with Perilipin Antibody NB110-40760AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.

Applications for Perilipin Antibody [Alexa Fluor® 488]

Application
Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Imaging Mass Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

50mM Sodium Borate

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: Perilipin

The perilipin family includes lipid droplet-associated protein (perilipin) and adipose differentiation-related protein (adipophilin). Perilipin is a modulator of both adipocyte lipid metabolism and adipophilinis involved in the development and maintenance of adipose tissue. Perilipin acts as a protective coating from the body's natural lipases--such as hormone-sensitive lipase--that break triglycerides into glycerol and free fatty acids for use in metabolism, a process called lipolysis. It is also an important regulator of lipid storage and its expression is elevated in obese animals and humans.

Alternate Names

PERI, PLIN, PLIN1

Gene Symbol

PLIN1

Additional Perilipin Products

Product Documents for Perilipin Antibody [Alexa Fluor® 488]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Perilipin Antibody [Alexa Fluor® 488]

Alexa Fluor (R) products are provided under an intellectual property license from Life Technologies Corporation. The purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment; (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Loading...
Loading...
Loading...
Loading...
Loading...
Loading...