Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Kappa Clone # POSTN/8523R

Format

Azide and BSA Free

Concentration

1 mg/ml

View all Periostin/OSF-2 Antibodies »

Product Specifications

Immunogen

Recombinant fragment (around aa193-326) of human Periostin/OSF-2 protein (exact sequence is proprietary)

Localization

Secreted. Extracellular space. Extracellular matrix.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG Kappa

Description

Positive Controls: Human placenta, breast or colon tissue.

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. Non-hazardous. No MSDS required.

Scientific Data Images for Periostin/OSF-2 Antibody (POSTN/8523R) - Azide and BSA Free

Periostin/OSF-2 Antibody (POSTN/8523R) - Azide and BSA Free Periostin/OSF-2 Antibody (POSTN/8523R) - Azide and BSA Free

Periostin/OSF-2 Antibody (POSTN/8523R) - Azide and BSA Free

Formalin-fixed, paraffin-embedded human colon stained with Periostin Recombinant Rabbit Monoclonal Antibody (POSTN/8523R). HIER: Tris/EDTA, pH9.0, 45min. 2: HRP-polymer, 30min. DAB, 5min.
Periostin/OSF-2 Antibody (POSTN/8523R) - Azide and BSA Free Periostin/OSF-2 Antibody (POSTN/8523R) - Azide and BSA Free

Periostin/OSF-2 Antibody (POSTN/8523R) - Azide and BSA Free

Formalin-fixed, paraffin-embedded human lung stained with Periostin Recombinant Rabbit Monoclonal Antibody (POSTN/8523R). Inset: PBS instead of primary antibody; secondary only negative control.

Applications for Periostin/OSF-2 Antibody (POSTN/8523R) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry-Paraffin

1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes. at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.

Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10mM PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -70C. Avoid freeze-thaw cycles.

Background: Periostin/OSF-2

Periostin is a disulfide linked 90 kDa 811 amno acid protein originally isolated as a osteoblastspecific factor that functions as a cell adhesion molecule for preosteoblasts and is thought to be involved in osteoblast recruitment, attachment and spreading. Additionally, periostin expression has previously been shown to be significantly increased by both transforming growth factor beta-1(TGFbeta1) and bone morphogenetic protein (BMP)-2. OSF-2 has a typical signal sequence, followed by a cysteine-rich domain, a fourfold repeated domain and a C-terminal domain. The fourfold repeated domain of OSF-2 shows homology with the insect protein fasciclin. Periostin mRNA is expressed in the developing mouse embryonic and fetal heart, and that it is localized to the endocardial cushions that ultimately divide the primitive heart tube into a four-chambered heart.

Long Name

Osteoblast Specific Factor 2

Alternate Names

Fasciclin I-like, OSF-2, OSF2, POSTN, TRIF52

Gene Symbol

POSTN

Additional Periostin/OSF-2 Products

Product Documents for Periostin/OSF-2 Antibody (POSTN/8523R) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Periostin/OSF-2 Antibody (POSTN/8523R) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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