Peripherin Antibody (7C5)

Western Blot: Peripherin Antibody (7C5) [NB300-220]

Western Blot: Peripherin Antibody (7C5) [NB300-220] - Analysis of tissue and cell lysates using Peripherin antibody, dilution 1:1,000 (Green): [1] protein standard, [2] rat spinal cord, [3] rat sciatic nerve, [4] mouse spinal cord, [5] pig spinal cord, [6] cow spinal cord, [7] SH-SY5Y cells, and [8] PC12 cells. The band at ~57kDa corresponds to the peripherin protein detected only in rat and mouse lysates, since the antibody is rodent specific.

Immunocytochemistry/ Immunofluorescence: Peripherin Antibody (7C5) [NB300-220]

Immunocytochemistry/Immunofluorescence: Peripherin Antibody (7C5) [NB300-220] - Analysis of mixed fibroblast and rat pheochromocytoma PC12 cell culture stained with peripherin antibody, dilution 1:500 (Red), and costained with chicken pAb to vimentin, dilution 1:10,000 (Green). Hoechst staining of nuclear DNA (Blue). The peripherin antibody reveals cytoplasmic filamentous staining in the small PC12 cells, while vimentin antibody stains intermediate filaments in the surrounding fibroblastic cells which do not express peripherin.

Immunocytochemistry/ Immunofluorescence: Peripherin Antibody (7C5) [NB300-220]

Immunocytochemistry/Immunofluorescence: Peripherin Antibody (7C5) [NB300-220] - Mixed neuron/glia cultures from newborn rat brain stained with NB300-220 antibody to peripherin (green) and rabbit polyclonal antibody to NF-L NBP1-05218 (red channel). A class of large neurons, like the one in the middle of this image, contain peripherin, while the majority of neurons and their processes contain NF-L and not peripherin. Interestingly, the periperin positive cells often contain a cytoplasmic inclusion next to the nucleus which stains for both peripherin and NF-L, and so appears golden in this kind of image. The blue channel reveals the localization of DNA.