Peripherin Antibody (8G2)

Western Blot: Peripherin Antibody (8G2) [NB300-138]

Western Blot: Peripherin Antibody (8G2) [NB300-138] - Analysis of tissue and cell lysates probed with mouse mAb to peripherin, NB300-138, dilution 1:500 in green: [1] protein standard (red), [2] mouse spinal cord, [3] rat spinal cord, [4] cow spinal cord, [5] SH-SY5Y cells, and [6] PC12 cells. The band at ~57kDa corresponds to the peripherin protein. Bands at 50 and 25kDa detected in the mouse spinal cord lysate, correspond to the heavy and light chains of mouse IgG.

Immunocytochemistry/ Immunofluorescence: Peripherin Antibody (8G2) [NB300-138]

Immunocytochemistry/Immunofluorescence: Peripherin Antibody (8G2) [NB300-138] - Analysis of human neuroblastoma cell line SH-SY5Y (A) and rat pheochromocytoma cell line PC12 (B), stained with mouse mAb to peripherin, NB300-138, dilution 1:500, in red, and costained with chicken pAb to vimentin, dilution 1:10,000, in green. The blue is DAPI staining of nuclear DNA. Peripherin, one of the Class III family of intermediate filament (IF) subunit proteins is revealed by NB300-138 antibody in the perinuclear region in some SH-SY5Y cells and in all PC12 cells. Vimentin, a protein also in the Class III IF family, is detected in a subpopulation of SH-SY5Y cells which are negative for peripherin. PC12 cells express peripherin but not vimentin.