Periplakin Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-55264

Novus Biologicals, part of Bio-Techne
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NB100-55264
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Key Product Details

Validated by

Independent Antibodies, Biological Validation

Species Reactivity

Human, Mouse, Rat

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

0.2 mg/ml

View all Periplakin Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 1150 and 1200 of human periplakin using the numbering given in entry NP_002696.3 (GeneID 5493).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Periplakin Antibody

Western Blot: Periplakin Antibody [NB100-55264]

Western Blot: Periplakin Antibody [NB100-55264]

Western Blot: Periplakin Antibody [NB100-55264] - Detection of Mouse and Rat Periplakin/PPL by Western Blot. Samples: Whole cell lysate (50 ug) from NIH3T3, TCMK-1, 4T1, CT26.WT, and rat C6 cells. Antibodies: Affinity purified rabbit anti-Periplakin/PPL antibody NB100-55264 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Western Blot: Periplakin Antibody [NB100-55264]

Western Blot: Periplakin Antibody [NB100-55264]

Western Blot: Periplakin Antibody [NB100-55264] - Detection of Human Periplakin/PPL on HeLa whole cell lysate using NB100-55264. Periplakin/PPL was also immunoprecipitated by another rabbit anti-Periplakin/PPL antibody.

Applications for Periplakin Antibody

Application
Recommended Usage

Immunoprecipitation

2-5 mcg/mg lysate

Western Blot

1:2000-1:10000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

TBS and 0.1% BSA

Preservative

0.09% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Periplakin

Peroplakin is a component of the cornified envelope of keratinocytes. It may link the cornified envelope to desmosomes and intermediate filaments and may act as a localization signal in PKB/AKT-mediated signaling. Endogenous periplakin localizes to different cellular compartments, including plasma membrane, intermediate filament structures, the nucleus and mitochondria. Overexpression of the C-terminal part of periplakin, encompassing the PKB binding region, results in predominant intermediate filament localization and little nuclear staining. Periplakin is expressed in epithelial cells, together with envoplakin, the precursor of the epidermal cornified envelope. Furthermore, periplakin has been shown to be a target antigen in paraneoplastic pemphigus, and in keratinocytes it localizes at desmosomes and the interdesmosomal plasma membrane.

Alternate Names

190 kDa paraneoplastic pemphigus antigen, 195 kDa cornified envelope precursor protein, KIAA0568, MGC134872, periplakin

Entrez Gene IDs

5493 (Human)

Gene Symbol

PPL

UniProt

O60437 (Human)

Additional Periplakin Products

Product Documents for Periplakin Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Periplakin Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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