Periplakin Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-94787

Novus Biologicals, part of Bio-Techne
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NBP2-94787-0.02ml
NBP2-94787-0.1ml
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Periplakin Antibodies »

Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-130 of human PPL (NP_002696.3). MNSLFRKRNKGKYSPTVQTRSISNKELSELIEQLQKNADQVEKNIVDTEAKMQSDLARLQEGRQPEHRDVTLQKVLDSEKLLYVLEADAAIAKHMKHPQGDMIAEDIRQLKERVTNLRGKHKQIYRLAVK

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

204 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Periplakin Antibody - BSA Free

Western Blot: Periplakin AntibodyBSA Free [NBP2-94787]

Western Blot: Periplakin AntibodyBSA Free [NBP2-94787]

Western Blot: Periplakin Antibody [NBP2-94787] - Analysis of extracts of various cell lines, using Periplakin antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 30s.
Immunocytochemistry/ Immunofluorescence: Periplakin Antibody - BSA Free [NBP2-94787]

Immunocytochemistry/ Immunofluorescence: Periplakin Antibody - BSA Free [NBP2-94787]

Immunocytochemistry/Immunofluorescence: Periplakin Antibody [NBP2-94787] - Analysis of mouse skin cells using PPL Rabbit pAb at dilution of 1:500 (40x lens). Blue: DAPI for nuclear staining.
Immunocytochemistry/ Immunofluorescence: Periplakin Antibody - BSA Free [NBP2-94787]

Immunocytochemistry/ Immunofluorescence: Periplakin Antibody - BSA Free [NBP2-94787]

Immunocytochemistry/Immunofluorescence: Periplakin Antibody [NBP2-94787] - Analysis of human skin cells using PPL Rabbit pAb at dilution of 1:500 (40x lens). Blue: DAPI for nuclear staining.

Applications for Periplakin Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100 - 1:500

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Periplakin

Peroplakin is a component of the cornified envelope of keratinocytes. It may link the cornified envelope to desmosomes and intermediate filaments and may act as a localization signal in PKB/AKT-mediated signaling. Endogenous periplakin localizes to different cellular compartments, including plasma membrane, intermediate filament structures, the nucleus and mitochondria. Overexpression of the C-terminal part of periplakin, encompassing the PKB binding region, results in predominant intermediate filament localization and little nuclear staining. Periplakin is expressed in epithelial cells, together with envoplakin, the precursor of the epidermal cornified envelope. Furthermore, periplakin has been shown to be a target antigen in paraneoplastic pemphigus, and in keratinocytes it localizes at desmosomes and the interdesmosomal plasma membrane.

Alternate Names

190 kDa paraneoplastic pemphigus antigen, 195 kDa cornified envelope precursor protein, KIAA0568, MGC134872, periplakin

Gene Symbol

PPL

Additional Periplakin Products

Product Documents for Periplakin Antibody - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Periplakin Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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