Peroxiredoxin 1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-06064

Novus Biologicals, part of Bio-Techne
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NBP1-06064
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Bovine (100%), Canine (100%), Mouse (100%), Porcine (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry-Paraffin, Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all Peroxiredoxin 1 Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-SDPKRTIAQDYG corresponding to internal region according to NP_859048.1.

Epitope

C-SDPKRTIAQDYG

Specificity

Reported variants represent identical protein (NP_002565.1, NP_859047.1, NP_859048.1).

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Peroxiredoxin 1 Antibody

Western Blot: Peroxiredoxin 1 Antibody [NBP1-06064]

Western Blot: Peroxiredoxin 1 Antibody [NBP1-06064]

Western Blot: Peroxiredoxin 1 Antibody [NBP1-06064] - Staining lysate of cell line HEK293 (A), A431 (B) and HepG2 (C). Antibody at 0.1 ug/mL. 35 ug protein in RIPA buffer. Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: Peroxiredoxin 1 Antibody [NBP1-06064]

Immunohistochemistry-Paraffin: Peroxiredoxin 1 Antibody [NBP1-06064]

Immunohistochemistry-Paraffin: Peroxiredoxin 1 Antibody [NBP1-06064] - Staining of paraffin embedded Human Small Intestine. Steamed antigen retrieval with citrate buffer Ph 6, AP-staining. Antibody at 2.5 ug/mL.

Applications for Peroxiredoxin 1 Antibody

Application
Recommended Usage

Immunohistochemistry-Paraffin

2.5 ug/mL

Peptide ELISA

Detection limit 1:8000

Western Blot

0.1 - 0.3 ug/mL
Application Notes
Western blot: Approx 24 kDa band observed in lysates of cell line HEK293, A431 and HepG2 (calculated MW of 22.1 kDa according to NP_859048.1).
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Peroxiredoxin 1

This gene encodes a member of the peroxiredoxin family of antioxidant enzymes, which reduce hydrogen peroxide and alkyl hydroperoxides. The encoded protein may play an antioxidant protective role in cells, and may contribute to the antiviral activity of CD8(+) T-cells. This protein may have a proliferative effect and play a role in cancer development or progression. Three transcript variants encoding the same protein have been identified for this gene. [provided by RefSeq]

Alternate Names

NKEF-A, PRDX1, PRX1, TDPX2

Entrez Gene IDs

5052 (Human); 18477 (Mouse); 117254 (Rat)

Gene Symbol

PRDX1

UniProt

NP_859048.1 (Human)

Additional Peroxiredoxin 1 Products

Product Documents for Peroxiredoxin 1 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Peroxiredoxin 1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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