Peroxiredoxin 2 Antibody (4E10-2D2)

Western Blot: Peroxiredoxin 2 Antibody (4E10-2D2) [H00007001-M01]

Western Blot: Peroxiredoxin 2 Antibody (4E10-2D2) [H00007001-M01] - Analysis of PRDX2 expression in transfected 293T cell line by PRDX2 monoclonal antibody (M01), clone 4E10-2D2.Lane 1: PRDX2 transfected lysate(21.9 KDa).Lane 2: Non-transfected lysate.

ELISA: Peroxiredoxin 2 Antibody (4E10-2D2) [H00007001-M01]

ELISA: Peroxiredoxin 2 Antibody (4E1.0-2D2) [H00007001-M01] - Peroxiredoxin 2 Antibody (4E10-2D2) [H00007001-M01] - Detection limit for recombinant GST tagged PRDX2 is approximately 1ng/ml as a capture antibody.

Knockdown Validated: Peroxiredoxin 2 Antibody (4E10-2D2) [H00007001-M01]

Knockdown Validated: Peroxiredoxin 2 Antibody (4E10-2D2) [H00007001-M01] - PRDX2 knockdown increases PRDX4 protein levels in HeLa cells. HeLa subclones were exposed to 20% or 1% O2 for indicated time. Each WCL was subject to immunoblot assays with the indicated antibodies (PRDX2 H00007001-M01 and PRDX4 NBP1-31435). Image collected and cropped by CiteAb from the following publication (oncotarget.com/abstract/7142), licensed under a CC-BY license.

Western Blot: Peroxiredoxin 2 Antibody (4E10-2D2) [H00007001-M01] -

Western Blot: Peroxiredoxin 2 Antibody (4E10-2D2) [H00007001-M01] - Expression of PRDX2 or PRDX4 does not affect HIF-1 alpha or HIF-2 alpha protein levelsA. & B. HeLa cells were transfected with EV or vector encoding PRDX2-V5 (A, P2) or PRDX4-V5 (B, P4), & exposed to 20% or 1% O2 for 24 h. WCL was subject to immunoblot assays with antibody against HIF-1 alpha, HIF-2 alpha, V5, or acin. C. HeLa-shSC (sc) & HeLa-shPRDX(2+4) (2+4) cells were exposed to 20% or 1% O2 for 24 h in the presence of doxycycline. WCL was subject to immunoblot assays with antibodies against HIF-1 alpha, HIF-2 alpha, PRDX2, PRDX4, & actin. Image collected & cropped by CiteAb from the following publication (https://www.oncotarget.com/lookup/doi/10.18632/oncotarget.7142), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: Peroxiredoxin 2 Antibody (4E10-2D2) [H00007001-M01] -

Western Blot: Peroxiredoxin 2 Antibody (4E10-2D2) [H00007001-M01] - PRDX2 knockdown increases PRDX4 protein levels in HeLa cellsHeLa subclones were exposed to 20% or 1% O2 for indicated time. Each WCL was subject to immunoblot assays with the indicated antibodies. SC, scrambled control shRNA. P2, PRDX2 shRNA. Image collected & cropped by CiteAb from the following publication (https://www.oncotarget.com/lookup/doi/10.18632/oncotarget.7142), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: Peroxiredoxin 2 Antibody (4E10-2D2) [H00007001-M01] -

Western Blot: Peroxiredoxin 2 Antibody (4E10-2D2) [H00007001-M01] - Hypoxia induces the nuclear translocation of PRDX2 & PRDX4HeLa cells were transfected with vector encoding PRDX2-V5 (P2) or PRDX4-V5 (P4), or empty vector (EV), & exposed to 20% or 1% O2 for 48 h. Nuclear & cytosolic fractions were isolated & subject to immunoblot assays with antibodies against HIF-1 alpha, HIF-2 alpha, V5, alpha-tubulin, & histone H3. Image collected & cropped by CiteAb from the following publication (https://www.oncotarget.com/lookup/doi/10.18632/oncotarget.7142), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: Peroxiredoxin 2 Antibody (4E10-2D2) [H00007001-M01] -

Western Blot: Peroxiredoxin 2 Antibody (4E10-2D2) [H00007001-M01] - Mapping the PRDX2 & PRDX4 binding domains of HIF-1 alphaA. & B. HeLa cells were transfected with PRDX2-V5 (A) or PRDX4-V5 (B) vector & WCL was incubated with purified GST or GST-HIF-1 alpha fusion protein in the presence of glutathione-Sepharose beads, followed by immunoblot assays with anti-V5 antibody (upper panels) or Ponceau S staining (lower panels). Image collected & cropped by CiteAb from the following publication (https://www.oncotarget.com/lookup/doi/10.18632/oncotarget.7142), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: Peroxiredoxin 2 Antibody (4E10-2D2) [H00007001-M01] -

Western Blot: Peroxiredoxin 2 Antibody (4E10-2D2) [H00007001-M01] - Expression of PRDX2 or PRDX4 does not affect HIF-1 alpha or HIF-2 alpha protein levelsA. & B. HeLa cells were transfected with EV or vector encoding PRDX2-V5 (A, P2) or PRDX4-V5 (B, P4), & exposed to 20% or 1% O2 for 24 h. WCL was subject to immunoblot assays with antibody against HIF-1 alpha, HIF-2 alpha, V5, or acin. C. HeLa-shSC (sc) & HeLa-shPRDX(2+4) (2+4) cells were exposed to 20% or 1% O2 for 24 h in the presence of doxycycline. WCL was subject to immunoblot assays with antibodies against HIF-1 alpha, HIF-2 alpha, PRDX2, PRDX4, & actin. Image collected & cropped by CiteAb from the following publication (https://www.oncotarget.com/lookup/doi/10.18632/oncotarget.7142), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: Peroxiredoxin 2 Antibody (4E10-2D2) [H00007001-M01] -

Western Blot: Peroxiredoxin 2 Antibody (4E10-2D2) [H00007001-M01] - Effect of PRDX2 & PRDX4 on HIF-1 alpha-p300 interactionHeLa cells were transfected with empty vector (EV) or vector encoding PRDX2-V5 or PRDX4-V5, & exposed to 1% O2 for 24 h. WCL was subject to IP with anti-p300 antibody, followed by immunoblot assays using antibodies against HIF-1 alpha, V5, & p300. Image collected & cropped by CiteAb from the following publication (https://www.oncotarget.com/lookup/doi/10.18632/oncotarget.7142), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: Peroxiredoxin 2 Antibody (4E10-2D2) [H00007001-M01] -

Western Blot: Peroxiredoxin 2 Antibody (4E10-2D2) [H00007001-M01] - PRDX2 expression is regulated by HIF-1 & HIF-2A. HeLa cells were exposed to 20% or 1% O2 for 24 h. RT-qPCR assays were performed using primers specific for the indicated mRNAs. Data are shown as mean ± SEM, n = 3. ***p < 0.001 versus 20% O2. B. HeLa cells were exposed to 20% or 1% O2 for the indicated time. WCLs were subject to immunoblot assays with antibodies against PRDX2, HIF-1 alpha, HIF-2 alpha, & actin. The PRDX2 & actin bands were quantified by densitometry & normalized to 0 h (20% O2). Normalized data are shown as mean ± SEM, n = 3. *p < 0.05, ***p < 0.001 versus 20% O2. C. HeLa-shSC (SC), HeLa-shHIF-1 alpha(1 alpha), HeLa-shHIF-2 alpha(2 alpha), & HeLa-sh1 alpha+2 alpha (DKD) cells were exposed to 20% or 1% O2 for 72 h. WCLs were subject to immunoblot assays with antibodies against PRDX2, HIF-1 alpha, HIF-2 alpha, & actin. Image collected & cropped by CiteAb from the following publication (https://www.oncotarget.com/lookup/doi/10.18632/oncotarget.7142), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: Peroxiredoxin 2 Antibody (4E10-2D2) [H00007001-M01] -

Western Blot: Peroxiredoxin 2 Antibody (4E10-2D2) [H00007001-M01] - PRDX proteins bind to HIF-1 alpha & HIF-2 alphaA. HeLa cells were transfected with an expression vector encoding V5-epitope-tagged PRDX2 (PRDX2-V5) & exposed to 1% O2 for 24 h. Whole cell lysate (WCL) was subject to immunoprecipitation (IP) using anti-HIF-1 alpha antibody or control IgG, followed by immunoblot assays with antibody against V5 epitope or HIF-1 alpha. B. HeLa cells were transfected with PRDX2-V5 vector & exposed to 1% O2 for 24 h. The WCL was subject to IP using anti-V5 antibody or control IgG, followed by immunoblot assays with antibody against V5 or HIF-1 alpha. Light IgG: immunoglobulin light chain from the secondary antibody. C. HeLa cells were transfected with vector encoding a V5-tagged PRDX family member & exposed to 1% O2 for 24 h. WCL was subject to IP using anti-HIF-1 alpha antibody, followed by immunoblot assays with antibody against V5 or HIF-1 alpha. D. HeLa cells were transfected with empty vector (EV) or vector encoding a V5-tagged PRDX family member & exposed to 1% O2 for 24 h. WCL was subject to IP using anti-HIF-2 alpha antibody, followed by immunoblot assays with antibody against V5 or HIF-2 alpha. Image collected & cropped by CiteAb from the following publication (https://www.oncotarget.com/lookup/doi/10.18632/oncotarget.7142), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: Peroxiredoxin 2 Antibody (4E10-2D2) [H00007001-M01] -

Western Blot: Peroxiredoxin 2 Antibody (4E10-2D2) [H00007001-M01] - PRDX proteins bind to HIF-1 alpha & HIF-2 alphaA. HeLa cells were transfected with an expression vector encoding V5-epitope-tagged PRDX2 (PRDX2-V5) & exposed to 1% O2 for 24 h. Whole cell lysate (WCL) was subject to immunoprecipitation (IP) using anti-HIF-1 alpha antibody or control IgG, followed by immunoblot assays with antibody against V5 epitope or HIF-1 alpha. B. HeLa cells were transfected with PRDX2-V5 vector & exposed to 1% O2 for 24 h. The WCL was subject to IP using anti-V5 antibody or control IgG, followed by immunoblot assays with antibody against V5 or HIF-1 alpha. Light IgG: immunoglobulin light chain from the secondary antibody. C. HeLa cells were transfected with vector encoding a V5-tagged PRDX family member & exposed to 1% O2 for 24 h. WCL was subject to IP using anti-HIF-1 alpha antibody, followed by immunoblot assays with antibody against V5 or HIF-1 alpha. D. HeLa cells were transfected with empty vector (EV) or vector encoding a V5-tagged PRDX family member & exposed to 1% O2 for 24 h. WCL was subject to IP using anti-HIF-2 alpha antibody, followed by immunoblot assays with antibody against V5 or HIF-2 alpha. Image collected & cropped by CiteAb from the following publication (https://www.oncotarget.com/lookup/doi/10.18632/oncotarget.7142), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: Peroxiredoxin 2 Antibody (4E10-2D2) [H00007001-M01] -

Western Blot: Peroxiredoxin 2 Antibody (4E10-2D2) [H00007001-M01] - PRDX2 expression is regulated by HIF-1 & HIF-2A. HeLa cells were exposed to 20% or 1% O2 for 24 h. RT-qPCR assays were performed using primers specific for the indicated mRNAs. Data are shown as mean ± SEM, n = 3. ***p < 0.001 versus 20% O2. B. HeLa cells were exposed to 20% or 1% O2 for the indicated time. WCLs were subject to immunoblot assays with antibodies against PRDX2, HIF-1 alpha, HIF-2 alpha, & actin. The PRDX2 & actin bands were quantified by densitometry & normalized to 0 h (20% O2). Normalized data are shown as mean ± SEM, n = 3. *p < 0.05, ***p < 0.001 versus 20% O2. C. HeLa-shSC (SC), HeLa-shHIF-1 alpha(1 alpha), HeLa-shHIF-2 alpha(2 alpha), & HeLa-sh1 alpha+2 alpha (DKD) cells were exposed to 20% or 1% O2 for 72 h. WCLs were subject to immunoblot assays with antibodies against PRDX2, HIF-1 alpha, HIF-2 alpha, & actin. Image collected & cropped by CiteAb from the following publication (https://www.oncotarget.com/lookup/doi/10.18632/oncotarget.7142), licensed under a CC-BY license. Not internally tested by Novus Biologicals.