Peroxiredoxin 2 Antibody (7F2-R)

Western Blot: Peroxiredoxin 2 Antibody (7F2-R) [NBP3-32778]

Western Blot: Peroxiredoxin 2 Antibody (7F2-R) [NBP3-32778] - Western blot analysis of Peroxiredoxin 2 on different lysates with Mouse anti-Peroxiredoxin 2 antibody (NBP3-32778) at 1/1,000 dilution.

Lane 1: HEK-293 cell lysate
Lane 2: LNCaP cell lysate
Lane 3: HeLa cell lysate
Lane 4: SH-SY5Y cell lysate
Lane 5: MCF7 cell lysate
Lane 6: HepG2 cell lysate

Lysates/proteins at 20 ug/Lane.

Predicted band size: 22 kDa
Observed band size: 22 kDa

Exposure time: 5 seconds;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32778) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.