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Peroxiredoxin 4 Antibody (PSH03-40)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-32739

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-32739

Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # PSH03-40

Concentration

1 mg/ml

Product Specifications

Immunogen

Recombinant protein within human Peroxiredoxin 4 aa 1-271 / 271. (Uniprot: Q13162)

Localization

Cytoplasm, Endoplasmic reticulum.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

31 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Peroxiredoxin 4 Antibody (PSH03-40)

Peroxiredoxin 4 Antibody (PSH03-40)

Western Blot: Peroxiredoxin 4 Antibody (PSH03-40) [NBP3-32739]

Western Blot: Peroxiredoxin 4 Antibody (PSH03-40) [NBP3-32739] - Western blot analysis of Peroxiredoxin 4 on different lysates with Rabbit anti-Peroxiredoxin 4 antibody (NBP3-32739) at 1/2,000 dilution.

Lane 1: HeLa cell lysate
Lane 2: HEK-293 cell lysate
Lane 3: HepG2 cell lysate
Lane 4: K-562 cell lysate
Lane 5: Jurkat cell lysate
Lane 6: MCF7 cell lysate
Lane 7: LNCaP cell lysate

Lysates/proteins at 20 ug/Lane.

Predicted band size: 31 kDa
Observed band size: 27 kDa

Exposure time: 50 seconds;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32739) at 1/2,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
Peroxiredoxin 4 Antibody (PSH03-40)

Immunohistochemistry: Peroxiredoxin 4 Antibody (PSH03-40) [NBP3-32739]

Immunohistochemistry: Peroxiredoxin 4 Antibody (PSH03-40) [NBP3-32739] - Immunohistochemical analysis of paraffin-embedded human breast tissue with Rabbit anti-Peroxiredoxin 4 antibody (NBP3-32739) at 1/500 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32739) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Peroxiredoxin 4 Antibody (PSH03-40)

Immunocytochemistry/ Immunofluorescence: Peroxiredoxin 4 Antibody (PSH03-40) [NBP3-32739]

Immunocytochemistry/ Immunofluorescence: Peroxiredoxin 4 Antibody (PSH03-40) [NBP3-32739] - Immunocytochemistry analysis of HeLa cells labeling Peroxiredoxin 4 with Rabbit anti-Peroxiredoxin 4 antibody (NBP3-32739) at 1/100 dilution.

Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Peroxiredoxin 4 antibody (NBP3-32739) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.

Applications for Peroxiredoxin 4 Antibody (PSH03-40)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry-Paraffin

1:500

Western Blot

1:2000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH7.4), 0.1% BSA and 40% Glycerol

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Peroxiredoxin 4

Peroxiredoxin-4 is encoded by this gene is an antioxidant enzyme and belongs to the peroxiredoxin family. The protein is localized to the cytoplasm. Peroxidases of the peroxiredoxin family reduce hydrogen peroxide and alkyl hydroperoxides to water and alcohol with the use of reducing equivalents derived from thiol-containing donor molecules. This protein has been found to play a regulatory role in the activation of the transcription factor NF-kappaB.

Alternate Names

AOE372, PRDX4, Prx4

Gene Symbol

PRDX4

Additional Peroxiredoxin 4 Products

Product Documents for Peroxiredoxin 4 Antibody (PSH03-40)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Peroxiredoxin 4 Antibody (PSH03-40)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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