Peroxiredoxin 5 Antibody (002)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-89862

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne

Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Knockout Validated, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 002

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Peroxiredoxin 5 Antibodies »

Product Specifications

Immunogen

This antibody was obtained from a rabbit immunized with purified, recombinant Human Peroxiredoxin 5 (Uniprot#: P30044-2; Met53-Leu214).

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for Peroxiredoxin 5 Antibody (002)

Western Blot: Peroxiredoxin 5 Antibody (002) [NBP2-89862]

Western Blot: Peroxiredoxin 5 Antibody (002) [NBP2-89862]

Western Blot: Peroxiredoxin 5 Antibody (002) [NBP2-89862] - Lane A: Hela Whole Cell Lysate Lane B: 293T Whole Cell LysateLane C: Jurkat Whole Cell Lysate Lane D: 293 Whole Cell LysateLysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:22 kDaObserved band size:15 kDa
Immunocytochemistry/ Immunofluorescence: Peroxiredoxin 5 Antibody (002) [NBP2-89862]

Immunocytochemistry/ Immunofluorescence: Peroxiredoxin 5 Antibody (002) [NBP2-89862]

Immunocytochemistry/Immunofluorescence: Peroxiredoxin 5 Antibody (002) [NBP2-89862] - Staining of PRDX5 in Hela cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with rabbit anti-human PRDX5 monoclonal antibody (1:60) at 4c overnight. Then cells were stained with the Alexa Fluor(R) 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).
Immunoprecipitation: Peroxiredoxin 5 Antibody (002) [NBP2-89862]

Immunoprecipitation: Peroxiredoxin 5 Antibody (002) [NBP2-89862]

Immunoprecipitation: Peroxiredoxin 5 Antibody (002) [NBP2-89862] - Lane A:0.5 mg Hela Whole Cell Lysate Lane B:0.5 mg Jurkat Whole Cell LysateLane C:0.5 mg 293T Whole Cell Lysate 2 uL anti-PRDX5 rabbit monoclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody:Anti-PRDX5 rabbit monoclonal antibody,at 1:500 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique. Performed under reducing conditions.Predicted band size: 22 kDa Observed band size: 22 kDa
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Applications for Peroxiredoxin 5 Antibody (002)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:20-1:100

Immunoprecipitation

4-8 uL/mg of lysate

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

0.2 um filtered solution in PBS

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Peroxiredoxin 5

Peroxiredoxin 5 encodes a member of the peroxiredoxin family of antioxidant enzymes, which reduce hydrogen peroxide and alkyl hydroperoxides. The encoded protein may play an antioxidant protective role in different tissues under normal conditions and during inflammatory processes. This protein interacts with peroxisome receptor 1. The crystal structure of this protein in its reduced form has been resolved to 1.5 angstrom resolution. This gene uses alternate in-frame translation initiation sites to generate mitochondrial or peroxisomal/cytoplasmic forms. Three transcript variants encoding distinct isoforms have been identified for this gene.

Alternate Names

ACR1, AOEB166, AOPP, PMP20, PRDX5, PRXV

Gene Symbol

PRDX5

Additional Peroxiredoxin 5 Products

Product Documents for Peroxiredoxin 5 Antibody (002)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Peroxiredoxin 5 Antibody (002)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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