Peroxiredoxin 5 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-38370

Novus Biologicals, part of Bio-Techne
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NBP2-38370
NBP2-38370-25ul
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Key Product Details

Validated by

Orthogonal Validation, Independent Antibodies

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Peroxiredoxin 5 Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: MGLAGVCALRRSAGYILVGGAGGQSAAAAARRCSEGEWASGGVRSFSRAAAAMAPIKVGDAIPAVEVFEGEPGN

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Peroxiredoxin 5 Antibody

Immunohistochemistry-Paraffin: Peroxiredoxin 5 Antibody [NBP2-38370]

Immunohistochemistry-Paraffin: Peroxiredoxin 5 Antibody [NBP2-38370]

Immunohistochemistry-Paraffin: Peroxiredoxin 5 Antibody [NBP2-38370] - Staining in human fallopian tube and liver tissues using anti-PRDX5 antibody. Corresponding PRDX5 RNA-seq data are presented for the same tissues.
Western Blot: Peroxiredoxin 5 Antibody [NBP2-38370]

Western Blot: Peroxiredoxin 5 Antibody [NBP2-38370]

Western Blot: Peroxiredoxin 5 Antibody [NBP2-38370] - Analysis using Anti-PRDX5 antibody NBP2-38370 (A) shows similar pattern to independent antibody NBP2-38371 (B).
Immunocytochemistry/ Immunofluorescence: Peroxiredoxin 5 Antibody [NBP2-38370]

Immunocytochemistry/ Immunofluorescence: Peroxiredoxin 5 Antibody [NBP2-38370]

Immunocytochemistry/Immunofluorescence: Peroxiredoxin 5 Antibody [NBP2-38370] - Immunofluorescent staining of human cell line U-2 OS shows localization to mitochondria.
View 2 more images

Applications for Peroxiredoxin 5 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Peroxiredoxin 5

Peroxiredoxin 5 encodes a member of the peroxiredoxin family of antioxidant enzymes, which reduce hydrogen peroxide and alkyl hydroperoxides. The encoded protein may play an antioxidant protective role in different tissues under normal conditions and during inflammatory processes. This protein interacts with peroxisome receptor 1. The crystal structure of this protein in its reduced form has been resolved to 1.5 angstrom resolution. This gene uses alternate in-frame translation initiation sites to generate mitochondrial or peroxisomal/cytoplasmic forms. Three transcript variants encoding distinct isoforms have been identified for this gene.

Alternate Names

ACR1, AOEB166, AOPP, PMP20, PRDX5, PRXV

Gene Symbol

PRDX5

UniProt

P30044

Additional Peroxiredoxin 5 Products

Product Documents for Peroxiredoxin 5 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Peroxiredoxin 5 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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