Peroxiredoxin 6 Antibody (1A9W5)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16369

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-16369-100ul
NBP3-16369-20ul
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 1A9W5

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Peroxiredoxin 6 Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 125-224 of human Peroxiredoxin 6 (NP_004896.1). KGMPVTARVVFVFGPDKKLKLSILYPATTGRNFDEILRVVISLQLTAEKRVATPVDWKDGDSVMVLPTIPEEEAKKLFPKGVFTKELPSGKKYLRYTPQP

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Peroxiredoxin 6 Antibody (1A9W5)

Western Blot: Peroxiredoxin 6 Antibody (1A9W5) [NBP3-16369]

Western Blot: Peroxiredoxin 6 Antibody (1A9W5) [NBP3-16369]

Western Blot: Peroxiredoxin 6 Antibody (1A9W5) [NBP3-16369] - Western blot analysis of extracts of various cell lines, using Peroxiredoxin 6 Rabbit mAb (NBP3-16369) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Applications for Peroxiredoxin 6 Antibody (1A9W5)

Application
Recommended Usage

Western Blot

1:500 - 1:1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Peroxiredoxin 6

FUNCTION: Involved in redox regulation of the cell. Can reduce H(2)O(2) and short chain organic, fatty acid, and phospholipid hydroperoxides. May play a role in the regulation of phospholipid turnover as well as in protection against oxidative injury. CATALYTIC ACTIVITY: 2 R'-SH + ROOH = R'-S-S-R' + H2O + ROH. SUBUNIT: Homotetramer. May interact with HTR2A. SUBCELLULAR LOCATION: Cytoplasm. Lysosome. Note=Also found in lung secretory organelles. MISCELLANEOUS: The active site is the redox-active Cys-47 oxidized to Cys-SOH. Cys-SOH may rapidly react with a Cys-SH of the other subunit to form an intermolecular disulfide with a concomitant homodimer formation. The enzyme may be subsequently regenerated by reduction of the disulfide by thioredoxin . MISCELLANEOUS: Irreversibly inactivated by overoxidation of Cys-47 (to Cys-SO(3)H) upon oxidative stress. SIMILARITY: Belongs to the ahpC/TSA family. Rehydrin subfamily.

Alternate Names

aiPLA2, AOP2, NSGPx, PRDX6

Gene Symbol

PRDX6

Additional Peroxiredoxin 6 Products

Product Documents for Peroxiredoxin 6 Antibody (1A9W5)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Peroxiredoxin 6 Antibody (1A9W5)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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