Peroxiredoxin 6 Antibody (22E7) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-59436

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Human

Applications

ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 22E7

Format

BSA Free

Concentration

1 mg/ml

View all Peroxiredoxin 6 Antibodies »

Product Summary for Peroxiredoxin 6 Antibody (22E7) - BSA Free

Immunogen

Recombinant human Peroxiredoxin 6 (1-224aa) purified from E. coli

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Scientific Data Images for Peroxiredoxin 6 Antibody (22E7) - BSA Free

Western Blot: Peroxiredoxin 6 Antibody (22E7)BSA Free [NBP2-59436]

Western Blot: Peroxiredoxin 6 Antibody (22E7)BSA Free [NBP2-59436]

Western Blot: Peroxiredoxin 6 Antibody (22E7) [NBP2-59436] - The recombinant human PRDX6 protein (50ng), Cell and Mouse tissue lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Peroxiredoxin 6 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: Recombinant PRDX6 protein Lane 2.: HeLa cell lysate Lane 3.: 293T cell lysate Lane 4.: HepG2 cell lysate Lane 5.: U87MG cell lysate Lane 6.: Ramos cell lysate
Immunocytochemistry/ Immunofluorescence: Peroxiredoxin 6 Antibody (22E7) - BSA Free [NBP2-59436]

Immunocytochemistry/ Immunofluorescence: Peroxiredoxin 6 Antibody (22E7) - BSA Free [NBP2-59436]

Immunocytochemistry/Immunofluorescence: Peroxiredoxin 6 Antibody (22E7) [NBP2-59436] - Analysis of PRDX6 in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human PRDX6 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).

Applications for Peroxiredoxin 6 Antibody (22E7) - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:1000

Western Blot

1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.4), 10% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Peroxiredoxin 6

FUNCTION: Involved in redox regulation of the cell. Can reduce H(2)O(2) and short chain organic, fatty acid, and phospholipid hydroperoxides. May play a role in the regulation of phospholipid turnover as well as in protection against oxidative injury. CATALYTIC ACTIVITY: 2 R'-SH + ROOH = R'-S-S-R' + H2O + ROH. SUBUNIT: Homotetramer. May interact with HTR2A. SUBCELLULAR LOCATION: Cytoplasm. Lysosome. Note=Also found in lung secretory organelles. MISCELLANEOUS: The active site is the redox-active Cys-47 oxidized to Cys-SOH. Cys-SOH may rapidly react with a Cys-SH of the other subunit to form an intermolecular disulfide with a concomitant homodimer formation. The enzyme may be subsequently regenerated by reduction of the disulfide by thioredoxin . MISCELLANEOUS: Irreversibly inactivated by overoxidation of Cys-47 (to Cys-SO(3)H) upon oxidative stress. SIMILARITY: Belongs to the ahpC/TSA family. Rehydrin subfamily.

Alternate Names

aiPLA2, AOP2, NSGPx, PRDX6

Gene Symbol

PRDX6

Additional Peroxiredoxin 6 Products

Product Documents for Peroxiredoxin 6 Antibody (22E7) - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Peroxiredoxin 6 Antibody (22E7) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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