PEX5 Antibody

Immunohistochemistry-Paraffin: PEX5 Antibody [NBP2-38443]

Immunohistochemistry-Paraffin: PEX5 Antibody [NBP2-38443] - Staining of human testis using Anti-PEX5 antibody NBP2-38443.

Western Blot: PEX5 Antibody [NBP2-38443]

Western Blot: PEX5 Antibody [NBP2-38443] - Analysis in U2OS cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-PEX5 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.

Immunohistochemistry-Paraffin: PEX5 Antibody [NBP2-38443]

Immunohistochemistry-Paraffin: PEX5 Antibody [NBP2-38443] - Staining of human pancreas shows low expression as expected.

Immunohistochemistry-Paraffin: PEX5 Antibody [NBP2-38443]

Immunohistochemistry-Paraffin: PEX5 Antibody [NBP2-38443] - Staining of human testis shows high expression.

Immunohistochemistry-Paraffin: PEX5 Antibody [NBP2-38443]

Immunohistochemistry-Paraffin: PEX5 Antibody [NBP2-38443] - Staining of human cerebral cortex, colon, lymph node and testis using Anti-PEX5 antibody NBP2-38443 (A) shows similar protein distribution across tissues to independent antibody NBP1-87185 (B).

Immunohistochemistry-Paraffin: PEX5 Antibody [NBP2-38443]

Immunohistochemistry-Paraffin: PEX5 Antibody [NBP2-38443] - Staining of human colon.

Immunohistochemistry-Paraffin: PEX5 Antibody [NBP2-38443]

Immunohistochemistry-Paraffin: PEX5 Antibody [NBP2-38443] - Staining of human lymph node.

Immunohistochemistry-Paraffin: PEX5 Antibody [NBP2-38443]

Immunohistochemistry-Paraffin: PEX5 Antibody [NBP2-38443] - Staining of human cerebral cortex.

Knockdown Validated: PEX5 Antibody [NBP2-38443]

Knockdown Validated: PEX5 Antibody [NBP2-38443] - PEX5 escorts ATGL to LD to mediate fasting-induced lipolysis. Western blot of whole cell extracts (WCE) or LD fractionation from adipocytes transfected with siNC or siPEX5. 30 ug of protein from WCE; 20 ug of protein from LD fraction. Image collected and cropped by CiteAb from the following publication (https://www.nature.com/articles/s41467-019-14176-0), licensed under a CC-BY license.

Western Blot: PEX5 Antibody [NBP2-38443] -

Western Blot: PEX5 Antibody [NBP2-38443] - PEX5 escorts ATGL to LD to mediate fasting-induced lipolysis.a Relative glycerol release from adipocytes transfected with negative control (NC) or PEX5 siRNA(siPEX5) for 48 h. n = 3 for each group. b Relative glycerol release from adipocytes transfected with siNC or siPEX5 for 48 h together in the absence or presence of WY-14643 (10 μM) treatment. n = 3 for each group. c Relative glycerol release from adipocytes transfected with siNC or siACOX1 for 48 h. n = 3 for each group. d, e Representative SIM images & quantification analysis of recruited ATGL to LDs in adipocytes immunostained with endogenous PLIN1 (red) & ATGL (green). Cells were transfected with siNC or siPEX5. n = 10 cells for siNC group; n = 15 cells treated with ISO; n = 12 cells for siPEX5 group; n = 13 cells for siPEX5 treated with ISO. Quantification of ATGL recruitment to LDs was measured using imageJ software. f Representative SIM z-section images (left) & fluorescence intensity profiles from the indicated line scans (right). Below 0.2 fluorescence intensity indicates background fluorescence signal. LD areas are highlighted in yellow. g Western blot of whole cell extracts (WCE) or LD fractionation from adipocytes transfected with siNC or siPEX5. 30 μg of protein from WCE; 20 μg of protein from LD fraction. h Quantification of ATGL in LDs normalized to PLIN1 from g. n = 4 independent experiments. CON control, ISO isoproterenol. Cells were treated with ISO (1 μM) for 1 h. All scale bars, 10 μm. Data represent the mean ± SD; *P < 0.05, ***P < 0.01 in two-way ANOVA followed by Turkey’s post-hoc test. n.s., not statistically significant. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/31996685), licensed under a CC-BY license. Not internally tested by Novus Biologicals.