PGRMC1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-83220

Novus Biologicals, part of Bio-Techne
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NBP1-83220
NBP1-83220-25ul
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Key Product Details

Validated by

Knockout/Knockdown, Orthogonal Validation

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human, Mouse, Rat

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Knockdown Validated, Simple Western, Western Blot

Cited:

Block/Neutralize, IF/IHC, Immunocytochemistry/ Immunofluorescence, Simple Western, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all PGRMC1 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: DQPAASGDSDDDEPPPLPRLKRRDFTPAELRRFDGVQDPRILMAINGKVFDVTKGRKFYGPEGPYGVFAGRDASR

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PGRMC1 Antibody

Western Blot: PGRMC1 Antibody [NBP1-83220]

Western Blot: PGRMC1 Antibody [NBP1-83220]

Western Blot: PGRMC1 Antibody [NBP1-83220] - Haem-dependent dimerization of PGRMC1 accelerates tumor growth through the EGFR signaling pathway. Nucleotide sequences of PGRMC1 targeted by shRNA and of the shRNA-resistant full length PGRMC1 expression vector. Stable PGRMC1-knockdown (PGRMC1-KD) HCT116 cells were transiently transfected with the shRNA-resistant expression vector of wild-type PGRMC1 (wt) or the Y113F mutant (Y113F). Image collected and cropped by CiteAb from the following publication (https://www.nature.com/articles/ncomms11030), licensed under a CC-BY license.
Immunohistochemistry-Paraffin: PGRMC1 Antibody [NBP1-83220]

Immunohistochemistry-Paraffin: PGRMC1 Antibody [NBP1-83220]

Immunohistochemistry-Paraffin: PGRMC1 Antibody [NBP1-83220] - Staining in human liver and skeletal muscle tissues . Corresponding PGRMC1 RNA-seq data are presented for the same tissues.
Western Blot: PGRMC1 Antibody [NBP1-83220]

Western Blot: PGRMC1 Antibody [NBP1-83220]

Western Blot: PGRMC1 Antibody [NBP1-83220] - Analysis in human cell line HEK 293.
View 11 more images

Applications for PGRMC1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

Reported in literature (PMID:23242527).

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500-1:1000

Simple Western

1:20

Western Blot

0.04-0.4 ug/ml
Application Notes
IHC-Paraffin HIER pH6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PGRMC1

Progesterone binding protein is a putative steroid membrane receptor. The protein is expressed predominantly in the liver and kidney.

Long Name

Progesterone Receptor Membrane Component 1

Alternate Names

HPR6.6, MPR, Progesterone Binding Protein

Gene Symbol

PGRMC1

Additional PGRMC1 Products

Product Documents for PGRMC1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for PGRMC1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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