Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse

Applications

Immunohistochemistry, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all PGRMC1 Antibodies »

Product Summary for PGRMC1 Antibody

Immunogen

The immunogen recognized by this antibody maps to a region between residue 145 to 195 of human Progesterone Receptor Membrane Component 1 using the numbering given in entry NP_006658.1.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PGRMC1 Antibody

Western Blot: PGRMC1 Antibody [NBP2-36525]

Western Blot: PGRMC1 Antibody [NBP2-36525]

Western Blot: PGRMC1 Antibody [NBP2-36525] - Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells prepared using NETN and RIPA lysis buffer. Antibodies: Affinity purified rabbit anti-PGRMC1 antibody used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposuretime of 30 seconds.
Immunohistochemistry: PGRMC1 Antibody [NBP2-36525]

Immunohistochemistry: PGRMC1 Antibody [NBP2-36525]

Immunohistochemistry: PGRMC1 Antibody [NBP2-36525] - Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti- PGRMC1 used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB. Counterstain: IHC Hematoxylin (blue).
Immunoprecipitation: PGRMC1 Antibody [NBP2-36525]

Immunoprecipitation: PGRMC1 Antibody [NBP2-36525]

Immunoprecipitation: PGRMC1 Antibody [NBP2-36525] - Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using RIPA lysis buffer. For blotting immunoprecipitated PGRMC1,it was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Applications for PGRMC1 Antibody

Application
Recommended Usage

Immunoprecipitation

2 - 10 ug/mg

Western Blot

1:1000 - 1:5000
Application Notes
Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
Validation by IP/Western Blot was performed using a 4-12% Bis-Tris gel.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: PGRMC1

Progesterone binding protein is a putative steroid membrane receptor. The protein is expressed predominantly in the liver and kidney.

Long Name

Progesterone Receptor Membrane Component 1

Alternate Names

HPR6.6, MPR, Progesterone Binding Protein

Gene Symbol

PGRMC1

UniProt

NP_006658.1 (Human)

Additional PGRMC1 Products

Product Documents for PGRMC1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for PGRMC1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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