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PHACTR4 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-82267

Novus Biologicals, part of Bio-Techne
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NBP1-82267-25ul
NBP1-82267

Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Mouse

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin

Cited:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: GRTRSLPITIEMLKVPDDEEEEEQTCPSTFSEEMTPTSVIPKLPQCLREEEEKESDSDSEGPIQYRDEEDEDESYQSALANKVKRKDTLAMKLNHRPSE

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 24748504). Rat (80%).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

78 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for PHACTR4 Antibody

Immunocytochemistry/ Immunofluorescence: PHACTR4 Antibody [NBP1-82267]

Immunocytochemistry/ Immunofluorescence: PHACTR4 Antibody [NBP1-82267]

Immunocytochemistry/Immunofluorescence: PHACTR4 Antibody [NBP1-82267] - Staining of human cell line A-431 shows localization to plasma membrane & cytosol. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: PHACTR4 Antibody [NBP1-82267]

Immunohistochemistry-Paraffin: PHACTR4 Antibody [NBP1-82267]

Immunohistochemistry-Paraffin: PHACTR4 Antibody [NBP1-82267] - Staining of human esophagus shows moderate cytoplasmic positivity in squamous epithelial cells.

Applications for PHACTR4 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF,Fixation/Permeabilization: PFA/Triton X-100

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PHACTR4

PHACTR4 encodes a member of the phosphatase and actin regulator (PHACTR) family. Other PHACTR family members have been shown to inhibit protein phosphatase 1 (PP1) activity, and the homolog of this gene in the mouse has been shown to interact with actin and PP1. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Alternate Names

DKFZp686L07205, FLJ13171, MGC20618, MGC34186, phosphatase and actin regulator 4

Entrez Gene IDs

65979 (Human)

Gene Symbol

PHACTR4

UniProt

Additional PHACTR4 Products

Product Documents for PHACTR4 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for PHACTR4 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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