Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse

Cited:

Human

Applications

Validated:

Immunoprecipitation, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all PHAX Antibodies »

Product Specifications

Immunogen

The immunogen this antibody was made to, maps to a region between residue 344 and 394 of human Phosphorylated Adaptor for RNA Export using the numbering given in entry NP_115553.2 (GeneID 51808).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PHAX Antibody

Western Blot: PHAX Antibody [NBP2-22268]

Western Blot: PHAX Antibody [NBP2-22268]

Western Blot: PHAX Antibody [NBP2-22268] - Association of NCBP3 and NCBP2 with NCBP1 and proteins involved in RNA processing. Western blot analysis of representative GFP-precipitated proteins identified by AP-MS analysis. Image collected and cropped by CiteAb from the following publication (https://www.nature.com/articles/ncomms9192), licensed under a CC-BY license.
Western Blot: PHAX Antibody [NBP2-22268]

Western Blot: PHAX Antibody [NBP2-22268]

Western Blot: PHAX Antibody [NBP2-22268] - Whole cell lysate from 293T (15 and 50 ug), HeLa (50 ug), and Jurkat (50 ug) cells. Antibodies: Affinity purified rabbit anti-PHAX antibody NBP2-22268 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
Western Blot: PHAX Antibody [NBP2-22268]

Western Blot: PHAX Antibody [NBP2-22268]

Western Blot: PHAX Antibody [NBP2-22268] - Whole cell lysate from mouse NIH3T3 (15 and 50 ug) cells. NBP2-22268 used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
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Applications for PHAX Antibody

Application
Recommended Usage

Immunoprecipitation

2 - 10 ug/mg

Western Blot

1:2000 - 1:10000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: PHAX

A phosphoprotein adapter involved in the XPO1-mediated U snRNA export from the nucleus. Bridge components required for U snRNA export, the cap binding complex (CBC)-bound snRNA on the one hand and the GTPase Ran in its active GTP-bound form together with the export receptor XPO1 on the other. Its phosphorylation in the nucleus is required for U snRNA export complex assembly and export, while its dephosphorylation in the cytoplasm causes export complex disassembly. It is recycled back to the nucleus via the importin alpha/beta heterodimeric import receptor. The directionality of nuclear export is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. Its compartmentalized phosphorylation cycle may also contribute to the directionality of export. Binds strongly to m7G-capped U1 and U5 small nuclear RNAs (snRNAs) in a sequence-unspecific manner and phosphorylation-independent manner . Plays also a role in the biogenesis of U3 small nucleolar RNA (snoRNA). Involved in the U3 snoRNA transport from nucleoplasm to Cajal bodies. Binds strongly to m7G-capped U3, U8 and U13 precursor snoRNAs and weakly to trimethylated (TMG)-capped U3, U8 and U13 snoRNAs. Binds also to telomerase RNA

Alternate Names

FLJ13193, phosphorylated adapter RNA export protein, phosphorylated adaptor for RNA export, RNA U small nuclear RNA export adapter protein, RNA U, small nuclear RNA export adapter (phosphorylation regulated), RNA U, small nuclear RNA export adaptor (phosphorylation regulated), RNUXA

Gene Symbol

PHAX

UniProt

NP_115553.2 (Human)

Additional PHAX Products

Product Documents for PHAX Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for PHAX Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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