PHC3 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-84896
Key Product Details
Species Reactivity
Validated:
Human, Mouse
Cited:
Mouse
Predicted:
Rat (94%). Backed by our 100% Guarantee.
Applications
Validated:
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Cited:
IF/IHC, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: GVLSSSQNGPPKSTSQTQSLTICHNKTTVTSSKISQRDPSPESNKKGESPSLESRSTAVTRTSSIHQLIAPASYSPIQPHSLIKHQQIPLHSPPSKVSHHQ
Reactivity Notes
Mouse reactivity reported in scientific literature (PMID: 23301003).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for PHC3 Antibody
![Western Blot: PHC3 Antibody [NBP1-84896]](https://resources.bio-techne.com/images/products/PHC3-Antibody-Western-Blot-NBP1-84896-img0005.jpg "Western Blot: PHC3 Antibody [NBP1-84896]")
Western Blot: PHC3 Antibody [NBP1-84896]
Western Blot: PHC3 Antibody [NBP1-84896] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp. Lane 4: Human plasma (IgG/HSA depleted). Lane 5: Human liver tissue. Lane 6: Human tonsil tissue
Immunohistochemistry-Paraffin: PHC3 Antibody [NBP1-84896] -
Immunohistochemistry-Paraffin: PHC3 Antibody [NBP1-84896] - Staining of human lymph node shows strong nuclear positivity in non-germinal center cells.
Immunohistochemistry-Paraffin: PHC3 Antibody [NBP1-84896] -
Immunohistochemistry-Paraffin: PHC3 Antibody [NBP1-84896] - Staining of human testis shows strong nuclear positivity in seminiferous ducts.Applications for PHC3 Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
Reported in scientific literature (PMID: 23301003)
Immunohistochemistry
1:200 - 1:500
Immunohistochemistry-Paraffin
1:200-1:500
Western Blot
0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended..
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: PHC3
Alternate Names
DKFZp313K1221, early development regulator 3, Early development regulatory protein 3, EDR3FLJ12729, FLJ12967, Homolog of polyhomeotic 3, hPH3, MGC88144, PH3, polyhomeotic homolog 3 (Drosophila), polyhomeotic like 3, polyhomeotic like 3 (Drosophila), polyhomeotic-like protein 3
Gene Symbol
PHC3
Additional PHC3 Products
Product Documents for PHC3 Antibody
Product Specific Notices for PHC3 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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