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Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Validated:

Human

Predicted:

Bovine (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 353 and 403 of human PHD finger protein 23 using the numbering given in entry NP_077273.2 (GeneID 79142).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PHF23 Antibody

Western Blot: PHF23 Antibody [NBP1-28747]

Western Blot: PHF23 Antibody [NBP1-28747]

Western Blot: PHF23 Antibody [NBP1-28747] - Samples: Whole cell lysate (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-PHF23 antibody NBP1-28747 used for WB at 0.1 ug/ml (A) and 0.4 ug/ml (B) and used for IP at 10 ug/mg lysate. PHF23 was also immunoprecipitated by rabbit anti-PHF23 antibody NBP1-28746, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A and B).
Immunohistochemistry: PHF23 Antibody [NBP1-28747]

Immunohistochemistry: PHF23 Antibody [NBP1-28747]

Immunohistochemistry: PHF23 Antibody [NBP1-28747] - Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti- PHF23 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB.

Applications for PHF23 Antibody

Application
Recommended Usage

Immunohistochemistry

1:500 - 1:2,000

Immunohistochemistry-Paraffin

1:500 - 1:2,000

Immunoprecipitation

10 ug/mg lysate

Western Blot

1:2000-1:10000
Application Notes
Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: PHF23

PHD finger protein 23 (PHF23) contains 1 PHD (plant homeodomain)-type zinc finger and has been identified in acute myeloid leukemia as part of a fusion gene involving NUP98.

Alternate Names

FLJ16355, FLJ22884, hJUNE-1b, MGC2941, PDH-containing protein JUNE-1, PHD finger protein 23, PHD finger protein 23a

Entrez Gene IDs

79142 (Human)

Gene Symbol

PHF23

UniProt

Additional PHF23 Products

Product Documents for PHF23 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for PHF23 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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