PHGDH Antibody (OTI5C4)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-46389
![Western Blot: PHGDH Antibody (OTI5C4) [NBP2-46389]](https://resources.bio-techne.com/images/products/PHGDH-Antibody-5C4-Western-Blot-NBP2-46389-img0002.jpg "Western Blot: PHGDH Antibody (OTI5C4) [NBP2-46389]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # OTI5C4
Concentration
1 mg/ml
Product Summary for PHGDH Antibody (OTI5C4)
Immunogen
Full length human recombinant protein of human PHGDH (NP_006614) produced in HEK293T cell.
Reactivity Notes
Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Scientific Data Images for PHGDH Antibody (OTI5C4)
Western Blot: PHGDH Antibody (OTI5C4) [NBP2-46389]
Western Blot: PHGDH Antibody (5C4) [NBP2-46389] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PHGDH.![Immunohistochemistry: PHGDH Antibody (OTI5C4) [NBP2-46389]](https://resources.bio-techne.com/images/products/PHGDH-Antibody-5C4-Immunohistochemistry-NBP2-46389-img0001.jpg "Immunohistochemistry: PHGDH Antibody (OTI5C4) [NBP2-46389]")
Immunohistochemistry: PHGDH Antibody (OTI5C4) [NBP2-46389]
Immunohistochemistry: PHGDH Antibody (5C4) [NBP2-46389] - Analysis of Human liver tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120 degrees C for 3min)Applications for PHGDH Antibody (OTI5C4)
Application
Recommended Usage
Immunohistochemistry
1:150
Immunohistochemistry-Paraffin
1:150
Western Blot
1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: PHGDH
Long Name
3-Phosphoglycerate Dehydrogenase
Alternate Names
3PGDH, PDGH3
Gene Symbol
PHGDH
Additional PHGDH Products
Product Documents for PHGDH Antibody (OTI5C4)
Product Specific Notices for PHGDH Antibody (OTI5C4)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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