PHGDH Antibody (OTI5C4) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-73372
![Western Blot: PHGDH Antibody (OTI5C4)Azide and BSA Free [NBP2-73372]](https://resources.bio-techne.com/images/products/PHGDH-Antibody-OTI5C4-Azide-and-BSA-Free-Western-Blot-NBP2-73372-img0001.jpg "Western Blot: PHGDH Antibody (OTI5C4)Azide and BSA Free [NBP2-73372]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # OTI5C4
Format
Azide and BSA Free
Concentration
LYOPH mg/ml
Product Summary for PHGDH Antibody (OTI5C4) - Azide and BSA Free
Immunogen
Full length human recombinant protein of human PHGDH (NP_006614) produced in HEK293T cell.
Reactivity Notes
Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Scientific Data Images for PHGDH Antibody (OTI5C4) - Azide and BSA Free
Western Blot: PHGDH Antibody (OTI5C4)Azide and BSA Free [NBP2-73372]
Western Blot: PHGDH Antibody (OTI5C4) - Azide and BSA Free [NBP2-73372] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PHGDH.
PHGDH Antibody (OTI5C4) - Azide and BSA Free [NBP2-73372] -
PHGDH Antibody (OTI5C4) - Azide and BSA Free [NBP2-73372] -Human liver tissue within the normal limits using anti-PHGDH mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120 C for 3min.Applications for PHGDH Antibody (OTI5C4) - Azide and BSA Free
Application
Recommended Usage
Immunohistochemistry
1:150
Immunohistochemistry-Paraffin
1:150
Western Blot
1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Reconstitution
we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.
Formulation
Lyophilized from PBS (pH 7.3) with 8% Trehalose
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
LYOPH mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: PHGDH
Long Name
3-Phosphoglycerate Dehydrogenase
Alternate Names
3PGDH, PDGH3
Gene Symbol
PHGDH
Additional PHGDH Products
Product Documents for PHGDH Antibody (OTI5C4) - Azide and BSA Free
Product Specific Notices for PHGDH Antibody (OTI5C4) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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