Phospho-Connexin 43/GJA1 (S368) Antibody

R&D Systems, part of Bio-Techne | Catalog # PPS046

R&D Systems, part of Bio-Techne
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PPS046
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Bovine, Canine, Chicken, Guinea Pig, Primate, Zebrafish

Cited:

Rat, Primate - Chlorocebus pygerythrus (Vervet Monkey)

Applications

Validated:

Western Blot

Cited:

Immunocytochemistry, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

View all Connexin 43/GJA1 Antibodies »

Product Specifications

Immunogen

Phosphopeptide corresponding to amino acid residues surrounding the phospho-S368 of Connexin 43/GJA1

Specificity

This antibody is specific for the 43 kDa Connexin 43/GJA1 in Western blots of rat brain lysates.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Phospho-Connexin 43/GJA1 (S368) Antibody

Detection of Phospho-Connexin 43/GJA1 (S368) antibody by Western Blot.

Detection of Phospho-Connexin 43/GJA1 (S368) by Western Blot.

Western blot of rat hippocampal lysate showing specific immunolabeling of the approximately 43 kDa Connexin 43/GJA1 phosphorylated at S368 (Control). The phosphospecificity of this labeling is demonstrated by treatment with 1200 units of lambda phosphatase ( lambda Phosphatase) for 30 minutes before being exposed to the Anti-Phospho-Connexin 43/GJA1 (S368). The immunolabeling of Connexin43 is completely eliminated by treatment with lambda PPase.

Applications for Phospho-Connexin 43/GJA1 (S368) Antibody

Application
Recommended Usage

Western Blot

1:1000 dilution
Sample: Rat brain (hippocampus) lysate

Reviewed Applications

Read 1 review rated 5 using PPS046 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

100 μL in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 μg/mL BSA and 50% glycerol.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

For long-term storage, ≤ ‑20° C is recommended. Product is stable at ≤ ‑20° C for at least 1 year.

Background: Connexin 43/GJA1

Connexin 43 (Cx43; also gap junction a-1 protein/GJA1) is a 41-44 kDa member of the connexin family, alpha-type subfamily, of transmembrane proteins. It is the most common type of connexin in cardiac muscle cells, and also occurs in hepatocytes, astrocytes and ovary granulosa cells. It is a 4-transmembrane protein 382 amino acids (aa) in length that contains two cytoplasmic tails. One is 12 aa in length at the N-terminus and the second is 151 aa in length at the C‑terminus. Human and rat Cx43 are 98% aa identical over the entire length of the molecule. Connexins form gap junctions (GJs) which are intercellular channels between cells. Each adjacent cell contributes to a functional channel. The fundamental unit is a “connexon”, or hemi-channel, which is composed of six connexins in a sliding subunit hexamer configuration. The hexamer may be either homomeric or heteromeric. The connexon arrangement provides for the opening and closing of an intersubunit space (or pore) that allows diffusion of molecules 1 kDa or less. It is suggested that the extended C-terminus of each connexin may interact with multiple docking proteins and serve as a plug during closure. Connexons on adjacent cells interact via their extracellular loops to form a GJ channel. When open, these channels allow for the transit of small hydrophilic molecules such as ATP, glucose, IP3 and Ca++. Connexons (or hemi-channels) that do not interact with adjacent cells are also suggested to allow for small molecule transit under unusual circumstances. Cx43 activity is regulated by phosphorylation. The exact effects, however, may be context-specific. In one case, PKC phosphorylation of S368 has been found to decrease GJ channel permeability to select small molecular weight solutes. Alternatively, PKA has been suggested to be the mediator of Cx43 phosphorylation of S365, S368, S369 and S373. In this case, phosphorylation promotes channel activity.

References

  1. Sohl, G. and K. Willecke (2004) Cardiovasc. Res. 62:228.
  2. Willecke, K. et al. (2002) Biol. Chem. 383:725.
  3. Giepmans, B.N.G. (2004) Cardiovasc. Res. 62:233.
  4. Vinken, M. et al. (2006) Cell. Signal. 18:592.
  5. Stout, C. et al. (2004) Curr. Opin. Cell Biol. 16:507.
  6. Bao, X. et al. (2004) Am. J. Physiol. Cell. Physiol. 286:C647.
  7. Yogo, K. et al. (2006) J. Reprod. Dev. 52:321.

Alternate Names

CX43, GJA1, GJAL, ODDD, ODOD, SDTY3

Entrez Gene IDs

2697 (Human)

Gene Symbol

GJA1

Additional Connexin 43/GJA1 Products

Product Documents for Phospho-Connexin 43/GJA1 (S368) Antibody

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Phospho-Connexin 43/GJA1 (S368) Antibody

For research use only

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