Phospho-Dynamin (S774) Antibody

Name: Phospho-Dynamin (S774) Antibody
Brand: R&D Systems
SKU: PPS004

R&D Systems, part of Bio-Techne | Catalog # PPS004

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Product Summary
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Bovine, Canine, Chicken, Primate

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG

View all Dynamin Antibodies »

Product Summary for Phospho-Dynamin (S774) Antibody

Immunogen

Phosphopeptide corresponding to amino acid residues surrounding phospho-S774 of Dynamin

Specificity

Human, mouse, rat, bovine, canine, chicken, and primate ~95 kDa Dynamin protein phosphorylated at S774. Labels the purified protein phosphorylated in vitro by cdk5 but not by PKC. Does not cross react with other purified substrates of cdk5 (e.g., Amphiphysin and Synapsin).

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Phospho-Dynamin (S774) Antibody

Detection of Phospho-Dynamin (S774) by Western Blot.

Western blot of rat brain (hippocampus) tissue lysate stimulated with forskolin showing specific immunolabeling of the ~95 kDa dynamin phosphorylated at S774 (Control). The phosphospecificity of this labeling is dem-onstrated by treatment with 1200 U of lambda Phosphatase ( lambda-PPase) for 30 minutes before being exposed to the Anti-Phospho-Dynamin (S774). The immunolabeling is completely eliminated by treatment with lambda-PPase.

Applications for Phospho-Dynamin (S774) Antibody

Application

Recommended Usage

Immunofluorescence

1:1000 dilution
Sample: Fixed culture neurons

Immunohistochemistry

1:1000 dilution
Sample: Fixed culture neurons

Western Blot

1:1000 dilution
Sample: Rat brain (hippocampus) tissue

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

100 μL in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 μg/mL BSA and 50% glycerol.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

For long-term storage, = -20° C is recommended. Product is stable at = -20° C for at least 1 year.

Background: Dynamin

Rat Dynamin (or Dynamin 1; also known as dephosphin) is a 95-100 kDa GTPase that belongs to the “classic dynamin” group of the dynamin superfamily. It is 864 amino acids (aa) in length, and contains three N-terminal GTP-binding elements (aa 38-208), one pleckstrin-homology domain (aa 519-625), a GED (GTPase effector domain) (aa 645-745), and a C-terminal SH3-binding proline-rich region (aa 753-864). At least four alternate splice forms are known, one that shows a 47 aa substitution between aa 398-445, and three that generate unique C-termini over the last 20 amino acids. Dynamin is found constitutively phosphorylated and inactive in the cytoplasm just under synaptic membranes. Upon stimulation, calcineurin dephosphorylates, and nitric oxide nitrosylates, Dynamin. Dephosphorylated and nitrosylated Dynamin now interacts with the cell membrane, generating endocytic synaptic vesicles. The process terminates when Cdk5 rephosphorylates Dynamin on S744 and S778.

Alternate Names

DNM1

Entrez Gene IDs

1759 (Human)

Gene Symbol