Phospho-STAT6 (Y641) PE-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # IC37173P
Recombinant Monoclonal Antibody.
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Intracellular Staining by Flow Cytometry
Label
Phycoerythrin (Excitation = 488 nm, Emission = 565-605 nm)
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 1248D
Product Specifications
Immunogen
Phosphopeptide containing human STAT6 Y641 site.
Accession # P42226
Accession # P42226
Specificity
Detects human STAT6 when phosphorylated at Y641.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Phospho-STAT6 (Y641) PE-conjugated Antibody
Detection of STAT6 in Human peripheral blood mononuclear cells (PBMCs) by Flow Cytometry.
Human peripheral blood mononuclear cells (PBMCs) either (A) Th2-stimulated or (B) unstimulated were stained with Rabbit Anti-Human Phospho-STAT6 (Y641) PE-conjugated Monoclonal Antibody (Catalog # IC37173P) and Mouse Anti-Human CD4 APC-conjugated Monoclonal Antibody (Catalog # FAB3791A). Quadrant markers were set based on control antibody staining (Catalog # IC1051P). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with methanol. View our protocol for Staining Intracellular Molecules.Applications for Phospho-STAT6 (Y641) PE-conjugated Antibody
Application
Recommended Usage
Intracellular Staining by Flow Cytometry
10 µL/106 cells
Sample: Th2-stimulated human peripheral blood mononuclear cells (PBMCs) fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with methanol
Sample: Th2-stimulated human peripheral blood mononuclear cells (PBMCs) fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with methanol
Formulation, Preparation, and Storage
Purification
Protein A or G purified from cell culture supernatant
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: STAT6
Long Name
Signal Transducer and Activator of Transcription 6
Alternate Names
D12S1644, EC 2.4.1.227, EC 2.7.7.6, IL-4 Stat, IL-4-STAT, signal transducer and activator of transcription 6, signal transducer and activator of transcription 6, interleukin-4 induced, STAT, interleukin-4 induced, STAT, interleukin4-induced, STAT6B, STAT6C, transcription factor IL-4 STAT
Gene Symbol
STAT6
UniProt
Additional STAT6 Products
Product Documents for Phospho-STAT6 (Y641) PE-conjugated Antibody
Product Specific Notices for Phospho-STAT6 (Y641) PE-conjugated Antibody
For research use only
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