Phospho-Synapsin I (S9) Antibody

R&D Systems, part of Bio-Techne | Catalog # PPS084

R&D Systems, part of Bio-Techne
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PPS084
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Key Product Details

Species Reactivity

Human, Mouse, Rat, Xenopus

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

View all Synapsin I Antibodies »

Product Specifications

Immunogen

Phosphopeptide corresponding to amino acid residues surrounding the phospho-S9 of Synapsin I

Specificity

Human, rat, mouse, and Xenopus ~78 kDa Synapsin I doublet protein phosphorylated at S9

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Phospho-Synapsin I (S9) Antibody

Detection of Phospho-Synapsin I (S9) antibody by Western Blot.

Detection of Phospho-Synapsin I (S9) by Western Blot.

Western blot of rat brain lysate showing specific immunolabeling of the approximately 78 kDa Synapsin I phosphorylated at S9 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase: lambda PPase). The blot is identical to the control except that it was incubated in lambda PPase (1200 units for 30 minutes) before being exposed to the anti-phospho-S9 Synapsin I. The immunolabeling is completely eliminated by treatment with lambda PPase.

Applications for Phospho-Synapsin I (S9) Antibody

Application
Recommended Usage

Western Blot

1:1000 dilution
Sample: Rat brain tissue

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

100 μL in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 μg/mL BSA and 50% glycerol.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

For long-term storage, ≤ -20° C is recommended. Product is stable at ≤ -20° C for at least 1 year.

Background: Synapsin I

Synapsin I is an 85 kDa neuron-specific, O-GlcNAcylated phosphoprotein that interacts with synaptic vesicles and regulates neurotransmitter release. It is concentrated in presynaptic terminals where it anchors synaptic vesicles to the underlying cytoskeleton. Phosphorylation at various sites reduces synapsin I binding to neurosecretory vesicles, making them available for neurotransmitter release. Rat synapsin I is 704 amino acids (aa) in length and contains five domains, named A - E/F. Domains A - D lie between aa 1 - 655, while E and F are alternate splice forms. Domain A (28 aa) regulates neurotransmitter release in a phosphorylation-dependent manner. Domains C (328 aa) and E (49 aa) anchor to actin in the synaptic region. Domains B (84 aa) and D (235 aa) promote actin and secretory vesicle interaction, and actin nucleation. Phosphorylation of S603 and 566 in domain D, and S9 in domain A, reduces the affinity of synapsin I for actin and secretory vesicles.

References

  1. Yamagata, Y. (2003) J. Pharmacol. Sci. 93:22.
  2. Sakurada, K. et al. (2002) J. Biol. Chem. 277:45473.
  3. Hilfiker, S. et al. (2005) J. Neurosci. 25:2658.
  4. McCaffery, C.A. and L.J. DeGennaro (1986) EMBO J. 5:3167.
  5. Cole, R.N. and G.W. Hart (1999) J. Neurochem. 73:418.

Alternate Names

SYN1

Entrez Gene IDs

6853 (Human); 20964 (Mouse); 24949 (Rat)

Gene Symbol

SYN1

Additional Synapsin I Products

Product Documents for Phospho-Synapsin I (S9) Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Phospho-Synapsin I (S9) Antibody

For research use only

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