Phospho-Synaptotagmin‑1 (T202) Antibody
R&D Systems, part of Bio-Techne | Catalog # PPS086
Key Product Details
Species Reactivity
Human, Mouse, Rat, Bovine, Canine, Chicken, Primate, Zebrafish
Applications
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Product Specifications
Immunogen
Phosphopeptide corresponding to amino acid residues surrounding the phospho-T202 of Synaptotagmin-1
Specificity
Rat Synaptotagmin phosphorylated at T202
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Phospho-Synaptotagmin‑1 (T202) Antibody
Detection of Phospho-Synaptotagmin-1 (T202) by Western Blot.
Western Blot of rat cortex lysate showing specific immunolabeling of the ~60 - 62 kDa Synaptotagmin phosphorylated at T202 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase; lambda PPase). The blot is identical to the control except that it was incubated in lambda PPase (1200 units for 30 minutes) before being exposed to anti-Synaptotagmin (T202). The immunolabeling is completely eliminated by treatment with lambda PPase.Applications for Phospho-Synaptotagmin‑1 (T202) Antibody
Application
Recommended Usage
Western Blot
1:1000 dilution
Sample: Rat brain tissue
Sample: Rat brain tissue
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
100 μL in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 μg/mL BSA, and 50% glycerol.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
For long-term storage, ≤ -20° C is recommended. Product is stable at ≤ -20° C for at least 1 year.
Background: Synaptotagmin-1
Synaptotagmins undergo three types of posttranslational modification that may affect function. N-linked glycosylation and/or O-linked glycosylation are likely necessary for recycling (internalization) of vesicle membrane after neurotransmitter release. Fatty acylation/palmitoylation of synaptotagmin may be necessary for proper cycling. Finally, synaptoagmin phosphorylation within the C2a site regulates calcium-binding, while phosphorylation in the C2b site may regulate calcium and syntaxin interaction.
Alternate Names
P65, SVP65, Synaptotagmin1, SYT1
Gene Symbol
SYT1
Additional Synaptotagmin-1 Products
Product Documents for Phospho-Synaptotagmin‑1 (T202) Antibody
Product Specific Notices for Phospho-Synaptotagmin‑1 (T202) Antibody
For research use only
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