Phospho-Tyrosine Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB16761
Key Product Details
Validated by
Biological Validation
Species Reactivity
Validated:
Multi-Species
Cited:
Insect - Drosophila
Applications
Validated:
Immunocytochemistry, Western Blot
Cited:
Immunohistochemistry-Frozen
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG2A Clone # 216954
Product Specifications
Immunogen
KLH-coupled Phospho-Tyrosine synthetic peptide
Specificity
Detects proteins containing phosphorylated tyrosine residues in Western blots. In Western blots of pervanadate-treated cell lysates, clone 216954 binds Phospho-Tyrosine in a broad manner largely independent of the surrounding amino acid sequence. No cross-reactivity with proteins or peptides containing phosphorylated serine or threonine residues is observed.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2A
Scientific Data Images for Phospho-Tyrosine Antibody
Detection of Phospho-Tyrosine by Western Blot.
Western blot shows lysates of A431 human epithelial carcinoma cell line untreated (-) or treated (+) with 50 µM pervanadate (PV) for 15 minutes. PVDF membrane was probed with 1 µg/mL of Rat Anti-Phospho-Tyrosine Monoclonal Antibody (Catalog # MAB16761), followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). Tyrosine-phosphorylated proteins were detected (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 10.Applications for Phospho-Tyrosine Antibody
Application
Recommended Usage
Immunocytochemistry
8-25 µg/mL
Sample: Immersion fixed A431 human epithelial carcinoma cell line treated with pervanadate
Sample: Immersion fixed A431 human epithelial carcinoma cell line treated with pervanadate
Western Blot
1 µg/mL
Sample: Pervanadate-treated A431 human epithelial carcinoma cell line
Sample: Pervanadate-treated A431 human epithelial carcinoma cell line
Reviewed Applications
Read 1 review rated 5 using MAB16761 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Phospho-Tyrosine
Alternate Names
PhosphoTyrosine
Additional Phospho-Tyrosine Products
Product Documents for Phospho-Tyrosine Antibody
Product Specific Notices for Phospho-Tyrosine Antibody
For research use only
Loading...
Loading...
Loading...
Loading...