Key Product Details

Validated by

Biological Validation

Species Reactivity

Non-species specific

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # G104

Concentration

1 mg/ml

View all Phospho-Tyrosine Antibodies »

Product Specifications

Immunogen

Phosphotyrosine, alanine and glyceine in a 1:1:1 ratio polymerized in the presence of keyhole limpet hemocyanin with 1-ethyl-3-(3'-dimentrylaminopropyl) carbodiimide

Specificity

Reacts with phosphotyrosine, and detects the presence of phosphotyrosine in both un-stimulated and stimulated cell lysates. Does not cross-react with phosphoserine or phosphothreonine.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Phospho-Tyrosine Antibody (G104)

Western Blot: Phospho-Tyrosine Antibody (G104) [NBP1-47562]

Western Blot: Phospho-Tyrosine Antibody (G104) [NBP1-47562]

Western Blot: Phospho-Tyrosine Antibody (G104) [NBP1-47562] - Western Blot analysis of Human A431 cell lysates showing detection of Phospho-Tyrosine protein using Mouse Anti-Phospho-Tyrosine Monoclonal Antibody, Clone G104 (NBP1-47562). Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Phospho-Tyrosine Monoclonal Antibody (NBP1-47562) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT. Left: normal, right: EGF treated.
Immunohistochemistry: Phospho-Tyrosine Antibody (G104) [NBP1-47562]

Immunohistochemistry: Phospho-Tyrosine Antibody (G104) [NBP1-47562]

Immunohistochemistry: Phospho-Tyrosine Antibody (G104) [NBP1-47562] - Immunohistochemistry analysis using Mouse Anti-Phospho-Tyrosine Monoclonal Antibody, Clone G104 (NBP1-47562). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-Phospho-Tyrosine Monoclonal Antibody (NBP1-47562) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Stratum granulosum staining in the epidermis. Some dermal staining.

Applications for Phospho-Tyrosine Antibody (G104)

Application
Recommended Usage

Immunohistochemistry

1:100

Immunohistochemistry-Paraffin

1:10-1:500

Western Blot

1:1000
Application Notes
1 ug/ml of Phosphotyrosine Antibody was sufficient for detection of phosphorylated tyrosine residues in 10 ug of rat tissue lysate by colorimetric immunoblot analysis using Goat anti-rat IgG:HRP as the secondary Antibody.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS (pH 7.4), 50% Glycerol

Preservative

0.09% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Phospho-Tyrosine

Phosphorylation is a common modification of proteins that can result in alterations in protein function, protein-protein association, cellular localization, and protein-half life. Phosphorylation can occur on threonine, serine, and tyrosine residues.

Alternate Names

PhosphoTyrosine

Additional Phospho-Tyrosine Products

Product Documents for Phospho-Tyrosine Antibody (G104)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Phospho-Tyrosine Antibody (G104)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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