Key Product Details

Species Reactivity

Non-species specific

Applications

Flow (Intracellular), Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # P-TYR-01

Format

BSA Free

Concentration

1 mg/ml

View all Phospho-Tyrosine Antibodies »

Product Specifications

Immunogen

Phosphotyrosine conjugated to BSA.

Reactivity Notes

Various species

Specificity

The antibody P-Tyr-01 detects tyrosine phosphorylation in activated cells.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Phospho-Tyrosine Antibody (P-TYR-01) - BSA Free

Immunocytochemistry/ Immunofluorescence: Phospho-Tyrosine Antibody (P-TYR-01) - BSA Free [NB500-335]

Immunocytochemistry/ Immunofluorescence: Phospho-Tyrosine Antibody (P-TYR-01) - BSA Free [NB500-335]

Immunocytochemistry/Immunofluorescence: Phospho-Tyrosine Antibody (P-TYR-01) [NB500-335] - Phosphotyrosine pattern in HeLa cells as revealed by P-Tyr-01 antibody-Alexa Fluor 488 (green). Actin detected by phalloidin-Alexa Fluor 567, DNA stained by DAPI (blue).

Applications for Phospho-Tyrosine Antibody (P-TYR-01) - BSA Free

Application
Recommended Usage

Flow (Intracellular)

2-5 ug/ml

Flow Cytometry

2-5 ug/ml

Immunocytochemistry/ Immunofluorescence

1:10-1:2000

Western Blot

1-2 ug/ml

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.4)

Format

BSA Free

Preservative

0.9% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Phospho-Tyrosine

The phosphorylation of specific tyrosine residues has been shown to be a primary mechanism of signal transduction during normal mitogenesis, cell cycle progression and oncogenic transformation, its role in other areas such as differentiation and gap junction communication, is a matter of active and ongoing research. Antibodies that specifically recognize phosphorylated tyrosine residues have proved to be invaluable to the study of tyrosine phosphorylated proteins and the biochemical pathways in which they function.

Alternate Names

PhosphoTyrosine

Additional Phospho-Tyrosine Products

Product Documents for Phospho-Tyrosine Antibody (P-TYR-01) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Phospho-Tyrosine Antibody (P-TYR-01) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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