Phospho-Tyrosine Biotinylated Antibody
R&D Systems, part of Bio-Techne | Catalog # BAM1676
Key Product Details
Validated by
Biological Validation
Species Reactivity
Validated:
Multi-Species
Cited:
Human, Rat
Applications
Validated:
ELISA, Western Blot
Cited:
ELISA Development (Detection), Western Blot
Label
Biotin
Antibody Source
Monoclonal Mouse IgG1 Clone # 179003
Product Specifications
Immunogen
KLH-coupled Phospho-Tyrosine synthetic peptide
Specificity
Detects proteins containing phosphorylated tyrosine residues. ELISA and 2D Western blot analyses using pervanadate-treated cell lysates indicate that clone 179003 binds Phospho-Tyrosine in a broad manner largely independent of the surrounding amino acid sequence. The antibody does not cross-react with proteins or peptides containing phosphorylated serine or threonine residues.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Phospho-Tyrosine Biotinylated Antibody
Detection of Phospho-PDGF R beta by ELISA.
Human Foreskin Fibroblasts were untreated or treated with 100 ng/mL Recombinant Human PDGF-AA (Catalog # 221-AA), Recombinant Human PDGF-AB (Catalog # 222-AB), or Recombinant Human PDGF-BB (Catalog # 222-BB) for 5 minutes at 37 °C in serum-free medium. Activation of PDGF R beta was assessed by ELISA using Human PDGF R beta Antibody (Catalog # MAB385) as the capture reagent and Phospho-Tyrosine Biotinylated Monoclonal Antibody (Catalog # BAM1676) as the detection reagent.
Detection of Phospho-Tyrosine by Western Blot.
Western blot shows lysates of A431 human epithelial carcinoma cell line untreated (-) or treated (+) with 50 µM pervanadate (PV) for 15 minutes. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Phospho-Tyrosine Biotinylated Monoclonal Antibody (Catalog # BAM1676), followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Tyrosine-phosphorylated proteins were detected (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 10.Applications for Phospho-Tyrosine Biotinylated Antibody
Application
Recommended Usage
ELISA
0.75 µg/mL
Sample: Proteins containing phosphorylated tyrosine residues
Sample: Proteins containing phosphorylated tyrosine residues
Western Blot
1 µg/mL
Sample: Pervanadate-treated A431 human epithelial carcinoma cell line
Sample: Pervanadate-treated A431 human epithelial carcinoma cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Phospho-Tyrosine
Alternate Names
PhosphoTyrosine
Additional Phospho-Tyrosine Products
Product Documents for Phospho-Tyrosine Biotinylated Antibody
Product Specific Notices for Phospho-Tyrosine Biotinylated Antibody
For research use only
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