Phosphodiesterase 4D Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-2462

Novus Biologicals, part of Bio-Techne
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NB100-2462
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Key Product Details

Species Reactivity

Human, Mouse

Applications

ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all Phosphodiesterase 4D Antibodies »

Product Specifications

Immunogen

Peptide with sequence QPEACVIDDRSPDT corresponding to C-Terminus according to NP_001184151.1, NP_001184150.1, NP_006194.2, NP_001098101.1, NP_001184152.1, NP_001159371.1.

Specificity

This antiobody is expected to recognize all reported isoforms.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Phosphodiesterase 4D Antibody

Western Blot: Phosphodiesterase 4D Antibody [NB100-2462]

Western Blot: Phosphodiesterase 4D Antibody [NB100-2462]

Western Blot: Phosphodiesterase 4D Antibody [NB100-2462] - HEK293 overexpressing Human PDE4D2 and probed with (mock transfection in first lane).
Immunohistochemistry-Paraffin: Phosphodiesterase 4D Antibody [NB100-2462]

Immunohistochemistry-Paraffin: Phosphodiesterase 4D Antibody [NB100-2462]

Immunohistochemistry-Paraffin: Phosphodiesterase 4D Antibody [NB100-2462] - staining of paraffin embedded Human Skeletal Muscle. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Western Blot: Phosphodiesterase 4D Antibody [NB100-2462]

Western Blot: Phosphodiesterase 4D Antibody [NB100-2462]

Western Blot: Phosphodiesterase 4D Antibody [NB100-2462] - (1ug/ml) of COS cell lysates (25ug protein): transfected with Human PDE4D1 (1), transfected with Human PDE4D3 (2), untransfected (3). Primary incubation was 1 hour. Detected by chemiluminescence.
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Applications for Phosphodiesterase 4D Antibody

Application
Recommended Usage

ELISA

1:200

Immunohistochemistry-Paraffin

5 ug/ml

Peptide ELISA

Detection limit 1:128000

Western Blot

0.1 - 0.3 ug/ml
Application Notes
ELISA successful usage is reported in scientific literature (PMID: 19939455). WB: Approx. 70+80+90 kDa band bands observed in lysates of A549. In transfected COS1 transiently expressing PDE4D1 and PDE4D3 bands of approx. 68 kDa and 95 kDa are observed. These bands are not observed in the untransfected COS1. In transfected HEK293 transiently expressing PDE4D2 multi bands from approx. 55 kDa to 80 kDa are observed. These bands are not observed in the non-transfected HEK293. Calculated MW of 91.1 kDa band according to PDE4D1 (NP_001098101.1), 76.5 kDa band according to PDE4D2 (NP_006194.2) and 84.7 kDa band according to PDE4D3 (NP_001159371.1).
IHC: Paraffin embedded Human Skeletal Muscle and Heart.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Phosphodiesterase 4D

Gene ID(s): 5144 (human)

Long Name

cAMP-specific 3',5'-cyclic phosphodiesterase 4D

Alternate Names

DPDE3, PDE43, PDE4D

Entrez Gene IDs

5144 (Human)

Gene Symbol

PDE4D

UniProt

NP_006194.2 (Human)

Additional Phosphodiesterase 4D Products

Product Documents for Phosphodiesterase 4D Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Phosphodiesterase 4D Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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