Phospholipase C beta 2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-13288

Novus Biologicals, part of Bio-Techne
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NBP3-13288
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Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Validated:

Human, Mouse

Predicted:

Rhesus Macaque (92%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Phospholipase C beta 2 Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human Phospholipase C beta 2. The exact sequence is proprietary.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

134 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Centrifuge briefly prior to opening.

Scientific Data Images for Phospholipase C beta 2 Antibody

Western Blot: Phospholipase C beta 2 Antibody [NBP3-13288]

Western Blot: Phospholipase C beta 2 Antibody [NBP3-13288]

Western Blot: Phospholipase C beta 2 Antibody [NBP3-13288] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membranes were blotted with Phospholipase C beta 2 antibody (NBP3-13288) diluted at 1:1000 and competitor's antibody diluted at 1:100. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Immunocytochemistry/ Immunofluorescence: Phospholipase C beta 2 Antibody [NBP3-13288]

Immunocytochemistry/ Immunofluorescence: Phospholipase C beta 2 Antibody [NBP3-13288]

Immunocytochemistry/Immunofluorescence: Phospholipase C beta 2 Antibody [NBP3-13288] - Phospholipase C beta 2 antibody detects Phospholipase C beta 2 protein at cytoplasm by immunofluorescent analysis. Sample: THP-1 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Phospholipase C beta 2 stained by Phospholipase C beta 2 antibody (NBP3-13288) diluted at 1:500. Blue: Fluoroshield with DAPI.
Immunohistochemistry-Paraffin: Phospholipase C beta 2 Antibody [NBP3-13288]

Immunohistochemistry-Paraffin: Phospholipase C beta 2 Antibody [NBP3-13288]

Immunohistochemistry-Paraffin: Phospholipase C beta 2 Antibody [NBP3-13288] - Human lung cancer. Phospholipase C beta 2 stained by Phospholipase C beta 2 antibody (NBP3-13288) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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Applications for Phospholipase C beta 2 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000
Application Notes
IHC-Fr Assay dependent

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS (pH 7), 20% Glycerol

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Phospholipase C beta 2

The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes

Long Name

1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-2

Alternate Names

EC 3.1.4.11, PLC-beta-2, PLCB2

Gene Symbol

PLCB2

Additional Phospholipase C beta 2 Products

Product Documents for Phospholipase C beta 2 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Phospholipase C beta 2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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