Phosphoserine Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NB100-1953
Key Product Details
Validated by
Biological Validation
Species Reactivity
Validated:
Non-species specific
Cited:
Human, Mouse
Applications
Validated:
ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot
Cited:
Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
0.25 mg/ml
Product Specifications
Immunogen
Phosphoserine conjugated to KLH, and phosvitin mixture
Specificity
Recognizes proteins phosphorylated on serine residues. Does not cross-react with phosphotyrosine.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Phosphoserine Antibody - BSA Free
Western Blot: Phosphoserine Antibody [NB100-1953]
Western Blot: Phosphoserine Antibody [NB100-1953] - Western blot analysis of Mouse Spleen lysates showing detection of Phosphoserineprotein using Rabbit Anti-PhosphoserinePolyclonal Antibody (NB100-1953). Primary Antibody: Rabbit Anti-PhosphoserinePolyclonal Antibody (NB100-1953) at 1:1000. Bands are responsive to treatment with varying long UV wavelengths: A(0), B(50), C(200), D(400), and E (treated with 0.1uM okadaic acid).Immunocytochemistry/ Immunofluorescence: Phosphoserine Antibody [NB100-1953]
Immunocytochemistry/Immunofluorescence: Phosphoserine Antibody [NB100-1953] - Tissue: HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-Phosphoserine Polyclonal Antibody at 1:50 for 12 hours at 4 degrees C. Secondary Antibody: R-PE Goat Anti-Rabbit (yellow) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Cytoplasm. Nucleus. Magnification: 100x.Western Blot: Phosphoserine Antibody [NB100-1953]
Western Blot: Phosphoserine Antibody [NB100-1953] - ~30 ug SY5Y or N2A whole cell lysate separated on 8% PAGE, blotted and probed with phosphoserine antibody (1:125 in 3% BSA in TBST). Western blot image submitted by a verified customer review.Applications for Phosphoserine Antibody - BSA Free
Application
Recommended Usage
ELISA
1:250
Immunocytochemistry/ Immunofluorescence
1:50
Immunohistochemistry
1:10 - 1:500
Immunohistochemistry-Paraffin
1:10 - 1:500
Immunoprecipitation
1:100
Western Blot
1:500
Application Notes
Phosphoserine antibody validated for WB from a verified customer review.
Reviewed Applications
Read 1 review rated 5 using NB100-1953 in the following applications:
Formulation, Preparation, and Storage
Purification
Peptide affinity purified
Formulation
PBS, 50% Glycerol
Format
BSA Free
Preservative
0.01 mg Sodium Azide
Concentration
0.25 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: Phosphoserine
Alternate Names
Phosphoserine; Phosphothreonine
Additional Phosphoserine Products
Product Documents for Phosphoserine Antibody - BSA Free
Product Specific Notices for Phosphoserine Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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