PI 3-Kinase p110 beta/PIK3CB Antibody (10D5)
Novus Biologicals, part of Bio-Techne | Catalog # H00005291-M03
Key Product Details
Species Reactivity
Human
Applications
ELISA, Immunocytochemistry/ Immunofluorescence, Proximity Ligation Assay, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2a Kappa Clone # 10D5
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
PIK3CB (NP_006210, 147 a.a. ~ 256 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. EFRRKMRKFSEEKILSLVGLSWMDWLKQTYPPEHEPSIPENLEDKLYGGKLIVAVHFENCQDVFSFQVSPNMNPIKVNELAIQKRLTIHGKEDEVSPYDYVLQVSGRVEY
Specificity
PIK3CB - phosphoinositide-3-kinase, catalytic, beta polypeptide (10D5)
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2a Kappa
Description
Quality control test: Antibody Reactive Against Recombinant Protein.
Scientific Data Images for PI 3-Kinase p110 beta/PIK3CB Antibody (10D5)
![Immunocytochemistry/ Immunofluorescence: PI 3-Kinase p110 beta/PIK3CB Antibody (10D5) [H00005291-M03]](https://resources.bio-techne.com/images/products/PI-3-Kinase-p110-beta-PIK3CB-Antibody-10D5-Immunocytochemistry-Immunofluorescence-H00005291-M03-img0003.jpg "Immunocytochemistry/ Immunofluorescence: PI 3-Kinase p110 beta/PIK3CB Antibody (10D5) [H00005291-M03]")
Immunocytochemistry/ Immunofluorescence: PI 3-Kinase p110 beta/PIK3CB Antibody (10D5) [H00005291-M03]
Immunocytochemistry/Immunofluorescence: PI 3-Kinase p110 beta/PIK3CB Antibody (10D5) [H00005291-M03] - Analysis of monoclonal antibody to PIK3CB on HeLa cell. Antibody concentration 20 ug/ml![Proximity Ligation Assay: PI 3-Kinase p110 beta/PIK3CB Antibody (10D5) [H00005291-M03]](https://resources.bio-techne.com/images/products/PI-3-Kinase-p110-beta-PIK3CB-Antibody-10D5-Proximity-Ligation-Assay-H00005291-M03-img0002.jpg "Proximity Ligation Assay: PI 3-Kinase p110 beta/PIK3CB Antibody (10D5) [H00005291-M03]")
Proximity Ligation Assay: PI 3-Kinase p110 beta/PIK3CB Antibody (10D5) [H00005291-M03]
Proximity Ligation Assay: PI 3-Kinase p110 beta/PIK3CB Antibody (10D5) [H00005291-M03] - Analysis of protein-protein interactions between PIK3R1 and PIK3CB HeLa cells were stained with anti-PIK3R1 rabbit purified polyclonal 1:1200 and anti-PIK3CB mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).Applications for PI 3-Kinase p110 beta/PIK3CB Antibody (10D5)
Application
Recommended Usage
Western Blot
1:500
Application Notes
Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB. GST tag alone is used as a negative control.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
IgG purified
Formulation
In 1x PBS, pH 7.4
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: PI 3-Kinase p110 beta
Long Name
Protein Tyrosine Phosphatase 1B
Alternate Names
PI 3Kinase p110 beta, PIK3CB
Entrez Gene IDs
5291 (Human)
Gene Symbol
PIK3CB
UniProt
Additional PI 3-Kinase p110 beta Products
Product Documents for PI 3-Kinase p110 beta/PIK3CB Antibody (10D5)
Product Specific Notices for PI 3-Kinase p110 beta/PIK3CB Antibody (10D5)
This product is produced by and distributed for Abnova, a company based in Taiwan.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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