PI 3-Kinase p110 gamma Antibody (2G3)

Novus Biologicals, part of Bio-Techne | Catalog # H00005294-M02

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Proximity Ligation Assay, Sandwich ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 2G3

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all PI 3-Kinase p110 gamma Antibodies »

Product Specifications

Immunogen

PIK3CG (AAH35683, 1 a.a. ~ 100 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MELENYKQPVVLREDNCRRRRRMKPRSAAASLSSMELIPIEFVLPTSQRKCKSPETALLHVAGHGNVEQMKAQVWLRALETSVAADFYHRLGPHHFLLLY

Specificity

PIK3CG (2G3)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for PI 3-Kinase p110 gamma Antibody (2G3)

Immunocytochemistry/ Immunofluorescence: PI 3-Kinase p110 gamma Antibody (2G3) [H00005294-M02]

Immunocytochemistry/ Immunofluorescence: PI 3-Kinase p110 gamma Antibody (2G3) [H00005294-M02]

Immunocytochemistry/Immunofluorescence: PI 3-Kinase p110 gamma Antibody (2G3) [H00005294-M02] - Analysis of monoclonal antibody to PIK3CG on HeLa cell . Antibody concentration 10 ug/ml.
Proximity Ligation Assay: PI 3-Kinase p110 gamma Antibody (2G3) [H00005294-M02]

Proximity Ligation Assay: PI 3-Kinase p110 gamma Antibody (2G3) [H00005294-M02]

Proximity Ligation Assay: PI 3-Kinase p110 gamma Antibody (2G3) [H00005294-M02] - Analysis of protein-protein interactions between KIT and PIK3CG. HeLa cells were stained with anti-KIT rabbit purified polyclonal 1:1200 and anti-PIK3CG mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).
Sandwich ELISA: PI 3-Kinase p110 gamma Antibody (2G3) [H00005294-M02] - Detection limit for recombinant GST tagged PIK3CG is approximately 10ng/ml as a capture antibody.

Applications for PI 3-Kinase p110 gamma Antibody (2G3)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB. GST tag alone is used as a negative control.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: PI 3-Kinase p110 gamma

This gene encodes a protein that belongs to the pi3/pi4-kinase family of proteins. The gene product is an enzyme that phosphorylates phosphoinositides on the 3-hydroxyl group of the inositol ring. It is an important modulator of extracellular signals, including those elicited by E-cadherin-mediated cell-cell adhesion, which plays an important role in maintenance of the structural and functional integrity of epithelia. In addition to its role in promoting assembly of adherens junctions, the protein is thought to play a pivotal role in the regulation of cytotoxicity in NK cells. The gene is located in a commonly deleted segment of chromosome 7 previously identified in myeloid leukemias.

Long Name

Protein Tyrosine Phosphatase 1G

Alternate Names

p120-PI3K, PI 3Kinase p110 gamma, PI3CG, PI3K-gamma, PIK3CG

Entrez Gene IDs

5294 (Human)

Gene Symbol

PIK3CG

OMIM

601232 (Human)

UniProt

AAH35683 (Human)

Additional PI 3-Kinase p110 gamma Products

Product Documents for PI 3-Kinase p110 gamma Antibody (2G3)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for PI 3-Kinase p110 gamma Antibody (2G3)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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