PI 3-Kinase p85 alpha Antibody (M253)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-23296

Novus Biologicals, part of Bio-Techne
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NBP3-23296
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Chicken

Applications

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # M253

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all PI 3-Kinase p85 alpha Antibodies »

Product Summary for PI 3-Kinase p85 alpha Antibody (M253)

Immunogen

Clone M253 was generated from a recombinant protein corresponding to amino acids in the C-terminal region of human PI 3-Kinase p85 alpha. This region is homologous to similar regions in rat and mouse PI 3-Kinase p85 alpha. [UniProt# P27986]

Specificity

This antibody detects an 85 kDa band corresponding to the p85 subunit of PI 3-Kinase in western blots of human A431 and Jurkat cells.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Theoretical MW

85 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Stable for at least 1 year at -20°C.

Scientific Data Images for PI 3-Kinase p85 alpha Antibody (M253)

PI 3-Kinase p85 alpha Antibody (M253)

Western Blot: PI 3-Kinase p85 alpha Antibody (M253) [NBP3-23296] -

Western Blot: PI 3-Kinase p85 alpha Antibody (M253) [NBP3-23296] - Western blot image of human A431 cells. The blots were probed with mouse monoclonal anti-PI 3-Kinase p85 alpha (C-terminal region) at 1:1000 (lane 1), 1:2000 (lane 2), and 1:4000 (lanes 3).
PI 3-Kinase p85 alpha Antibody (M253)

Immunocytochemistry/Immunofluorescence: PI 3-Kinase p85 alpha Antibody (M253) [NBP3-23296] -

Immunocytochemistry/Immunofluorescence: PI 3-Kinase p85 alpha Antibody (M253) [NBP3-23296] - Immunocytochemical labeling of PI 3-Kinase p85 alpha in aldehyde-fixed and NP-40-permeabilized A431 cells. The cells were labeled with mouse monoclonal PI 3-Kinase p85 alpha (C-terminal region) antibody (NBP3-23296), then the antibody was detected using appropriate secondary antibody conjugated to DyLight(R) 594.

Applications for PI 3-Kinase p85 alpha Antibody (M253)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Western Blot

1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS, 1 mg/ml BSA and 50% glycerol

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C.

Background: PI 3-Kinase p85 alpha

Phosphatidylinositol 3-kinase (PI3K) p85 alpha is a regulatory subunit that heterodimerizes with a catalytic subunit to form a Class IA PI3K enzyme complex, which plays an important role in the immune system (1,2). The P13K pathway is involved in many diverse processes including growth, metabolism, proliferation, and survival (2). PI3Ks are typically activated by cytokine receptors and are responsible for phosphorylation of the 3'-hydroxyl group of PI and its derivatives (3). p85 alpha is one of five regulatory subunit proteins which also includes p55 alpha, p50 alpha, p85 beta, and p55 gamma, and can bind to two of the three catalytic subunits (p110 alpha or p110 delta) (1,2). p85 alpha, p55 alpha, and p50 alpha proteins are all synthesized by the same PIK3R1 gene by alternative splicing (1). Structurally, PI 3-Kinase p85 alpha contains SRC homology 3 (SH3 domain), followed by a Bcr homology (BH) domain flanked by two proline-rich regions, then a N-terminal SH2 domain, an inter-SH2 domain, and C-terminal SH2 domain (1,2). PI 3-Kinase p85 alpha protein consists of 724 amino acids (aa) in length with a theoretical molecular weight of 83.5 kDa (2,4). The primary role for the p85 subunit is interaction with cell surface receptors and acts as an adapter for the stabilization and recruitment of the p110 catalytic subunit to the plasma membrane (1,3). Additionally, p85 has been shown to function in both interleukin-2 receptor (IL2R) and erythropoietin receptor (EpoR) endocytosis (3).

The PI3K pathway functions in a broad range of cellular processes, so it is understandable that pathway dysfunction can lead to an array of diseases and disorders (2,5). Elevated PI3K signaling is a key feature of many cancers (5). PI3K pathway dysregulation has also been implicated in neurological, metabolic, and cardiovascular disorders (5). Furthermore, both overactivation or under-activation of the PI3K delta (p85 alpha subunit + p110 delta subunit) pathway has been shown to cause immunodeficiency and pathologies related to immune system dysfunction (2). Therapeutics to target the PI3K pathway and treat related cancers include PI3K inhibitors and, specifically, isoform-selective inhibitors which have a lot of promise when used as part of a combination therapy (5).

References

1. Okkenhaug, K., & Vanhaesebroeck, B. (2001). New responsibilities for the PI3K regulatory subunit p85 alpha. Science's STKE : signal transduction knowledge environment. https://doi.org/10.1126/stke.2001.65.pe1

2. Nunes-Santos, C. J., Uzel, G., & Rosenzweig, S. D. (2019). PI3K pathway defects leading to immunodeficiency and immune dysregulation. The Journal of allergy and clinical immunology. https://doi.org/10.1016/j.jaci.2019.03.017

3. Chen, P. H., Yao, H., & Huang, L. J. (2017). Cytokine Receptor Endocytosis: New Kinase Activity-Dependent and -Independent Roles of PI3K. Frontiers in endocrinology. https://doi.org/10.3389/fendo.2017.00078

4. Uniprot (P27986)

5. Fruman, D. A., Chiu, H., Hopkins, B. D., Bagrodia, S., Cantley, L. C., & Abraham, R. T. (2017). The PI3K Pathway in Human Disease. Cell. https://doi.org/10.1016/j.cell.2017.07.029

Long Name

PI 3-Kinase p85 alpha

Alternate Names

GRB1, PI 3Kinase p85 alpha, PIK3R1

Gene Symbol

PIK3R1

Additional PI 3-Kinase p85 alpha Products

Product Documents for PI 3-Kinase p85 alpha Antibody (M253)

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Product Specific Notices for PI 3-Kinase p85 alpha Antibody (M253)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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