PI 3-Kinase p85 beta Antibody (6E2F5)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16493
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 6E2F5
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 100-200 of human PI 3-Kinase p85 beta (O00459). PRDGAPEPGLTLPDLPEQFSPPDVAPPLLVKLVEAIERTGLDSESHYRPELPAPRTDWSLSDVDQWDTAALADGIKSFLLALPAPLVTPEASAEARRALRE
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for PI 3-Kinase p85 beta Antibody (6E2F5)
Western Blot: PI 3-Kinase p85 beta Antibody (6E2F5) [NBP3-16493]
Western Blot: PI 3-Kinase p85 beta Antibody (6E2F5) [NBP3-16493] - Western blot analysis of extracts of various cell lines, using PI 3-Kinase p85 beta antibody (NBP3-16493) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.Immunohistochemistry-Paraffin: PI 3-Kinase p85 beta Antibody (6E2F5) [NBP3-16493]
Immunohistochemistry-Paraffin: PI 3-Kinase p85 beta Antibody (6E2F5) [NBP3-16493] - Immunohistochemistry of paraffin-embedded mouse testis using PI 3-Kinase p85 beta Rabbit mAb (NBP3-16493) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.Immunohistochemistry-Paraffin: PI 3-Kinase p85 beta Antibody (6E2F5) [NBP3-16493]
Immunohistochemistry-Paraffin: PI 3-Kinase p85 beta Antibody (6E2F5) [NBP3-16493] - Immunohistochemistry of paraffin-embedded rat testis using PI 3-Kinase p85 beta Rabbit mAb (NBP3-16493) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.Applications for PI 3-Kinase p85 beta Antibody (6E2F5)
Application
Recommended Usage
Immunohistochemistry
1:50 - 1:200
Western Blot
1:500 - 1:1000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS, 0.05% BSA, 50% glycerol, pH7.3
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: PI 3-Kinase p85 beta
Long Name
Phosphatidylinositol-3-kinase 85 kDa Regulatory Subunit beta
Alternate Names
p85-beta, PI 3Kinase p85 beta, PIK3R2
Gene Symbol
PIK3R2
Additional PI 3-Kinase p85 beta Products
Product Documents for PI 3-Kinase p85 beta Antibody (6E2F5)
Product Specific Notices for PI 3-Kinase p85 beta Antibody (6E2F5)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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