PI 3-Kinase p85 beta Antibody (PIK3R2/292) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-14138
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Applications
Immunohistochemistry-Paraffin, Protein Array
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2b Kappa Clone # PIK3R2/292
Format
Azide and BSA Free
Concentration
1 mg/ml
Product Summary for PI 3-Kinase p85 beta Antibody (PIK3R2/292) - Azide and BSA Free
Immunogen
Recombinant fragment of human PI 3-Kinase p85 beta protein (around aa 520-720) (exact sequence is proprietary) (Uniprot: O00459)
Localization
Cytoplasm,golgi apparatus, Nucleus
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2b Kappa
Theoretical MW
85 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP3-13735).
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
Scientific Data Images for PI 3-Kinase p85 beta Antibody (PIK3R2/292) - Azide and BSA Free
Protein Array: PI 3-Kinase p85 beta Antibody (PIK3R2/292) - Azide and BSA Free [NBP3-14138]
Protein Array: PI 3-Kinase p85 beta Antibody (PIK3R2/292) - Azide and BSA Free [NBP3-14138] - Analysis of Protein Array containing more than 19,000 full-length human proteins using PI 3-Kinase p85 beta Antibody (PIK3R2/292).PI 3-Kinase p85 beta Antibody (PIK3R2/292)
Formalin-fixed, paraffin-embedded human spleen stained with PI 3-Kinase p85 beta antibody (PIK3R2/292). HIER: Tris/EDTA, pH9.0, 45min. Secondary: HRP-polymer, 30min. DAB, 5min.Applications for PI 3-Kinase p85 beta Antibody (PIK3R2/292) - Azide and BSA Free
Application
Recommended Usage
Immunohistochemistry-Paraffin
1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10mM PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Background: PI 3-Kinase p85 beta
Long Name
Phosphatidylinositol-3-kinase 85 kDa Regulatory Subunit beta
Alternate Names
p85-beta, PI 3Kinase p85 beta, PIK3R2
Gene Symbol
PIK3R2
Additional PI 3-Kinase p85 beta Products
Product Documents for PI 3-Kinase p85 beta Antibody (PIK3R2/292) - Azide and BSA Free
Product Specific Notices for PI 3-Kinase p85 beta Antibody (PIK3R2/292) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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