Key Product Details

Species Reactivity

Bovine

Applications

Immunohistochemistry, Immunohistochemistry-Frozen, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # T15

Concentration

1.0 mg/ml

View all PI 3-Kinase p85 beta Antibodies »

Product Specifications

Immunogen

Balculovirus expressed (Recombinant) p85 beta subunit of bovine PI-3 kinase

Reactivity Notes

Predicted cross-reactivities: Human, Monkey

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Specificity

Recognizes the p85 subunit of bovine PI-3 kinase.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for PI 3-Kinase p85 beta Antibody (T15)

Western Blot: PI 3-Kinase p85 beta Antibody (T15)BSA Free [NB100-65590]

Western Blot: PI 3-Kinase p85 beta Antibody (T15)BSA Free [NB100-65590]

Western Blot: PI 3-Kinase p85 beta Antibody (T15) [NB100-65590] - Analysis of HEK293 and HepG2 cell lysates with Mouse anti Bovine PI-3 kinase p85 subunit beta, recombinant p85beta is included as a positive control.

Applications for PI 3-Kinase p85 beta Antibody (T15)

Application
Recommended Usage

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Frozen

1:10-1:500

Immunoprecipitation

1:10-1:500

Western Blot

1:100-1:2000

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PI 3-Kinase p85 beta

The enzyme phosphatidylinositol 3 kinase (PI3 kinase) is a lipid kinase that generates phosphatidylinositol 3, 4, 5-triphosphate in response to receptor activation in many signal transduction pathways. Class IA PI3Ks exist as a heterodimer of a catalytic 110 kDa (p110) and a regulatory p85 subunit (e.g. p85 beta). p85 beta is an adaptor molecule that regulates the activity of the catalytic p110 subunit by binding to phosphorylated receptor tyrosine kinases (RTKs) through its SH2 domain and mediating the interaction between p110 and the plasma membrane. p85 beta is necessary for insulin-stimulated increase in glucose uptake and glycogen synthesis in insulin-sensitive tissues.

Long Name

Phosphatidylinositol-3-kinase 85 kDa Regulatory Subunit beta

Alternate Names

p85-beta, PI 3Kinase p85 beta, PIK3R2

Entrez Gene IDs

5296 (Human); 18709 (Mouse); 29741 (Rat)

Gene Symbol

PIK3R2

UniProt

P23726 (Bovine)

Additional PI 3-Kinase p85 beta Products

Product Documents for PI 3-Kinase p85 beta Antibody (T15)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for PI 3-Kinase p85 beta Antibody (T15)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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