PI 3-Kinase p85 beta Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-41066

Novus Biologicals, part of Bio-Techne
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NBP1-41066
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Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunoprecipitation, Western Blot

Cited:

IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

0.2 mg/ml

View all PI 3-Kinase p85 beta Antibodies »

Product Specifications

Immunogen

The immunogen for this product maps to a region between residue 75 and 125 of human phosphoinositide-3-kinase, regulatory subunit 2 using the numbering given in entry NP_005018.1 (GeneID 5296).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PI 3-Kinase p85 beta Antibody

Western Blot: PI 3-Kinase p85 beta Antibody [NBP1-41066]

Western Blot: PI 3-Kinase p85 beta Antibody [NBP1-41066]

Western Blot: PI 3-Kinase p85 beta Antibody [NBP1-41066] - Whole cell lysate (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Affinity purified rabbit anti-PIK3R2 antibody used for WB at 0.04 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 10 mcg/mg lysate. PIK3R2 was also immunoprecipitated by rabbit anti-PIK3R2 antibody NBP1-41067, which recognizes a downstream epitope.

Applications for PI 3-Kinase p85 beta Antibody

Application
Recommended Usage

Immunoprecipitation

5-15 ug/mg lysate

Western Blot

1:2000-1:10000
Application Notes
Use in Immunohistochemistry reported in scientific literature (PMID 27096956)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

TBS and 0.1% BSA

Preservative

0.09% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: PI 3-Kinase p85 beta

The enzyme phosphatidylinositol 3 kinase (PI3 kinase) is a lipid kinase that generates phosphatidylinositol 3, 4, 5-triphosphate in response to receptor activation in many signal transduction pathways. Class IA PI3Ks exist as a heterodimer of a catalytic 110 kDa (p110) and a regulatory p85 subunit (e.g. p85 beta). p85 beta is an adaptor molecule that regulates the activity of the catalytic p110 subunit by binding to phosphorylated receptor tyrosine kinases (RTKs) through its SH2 domain and mediating the interaction between p110 and the plasma membrane. p85 beta is necessary for insulin-stimulated increase in glucose uptake and glycogen synthesis in insulin-sensitive tissues.

Long Name

Phosphatidylinositol-3-kinase 85 kDa Regulatory Subunit beta

Alternate Names

p85-beta, PI 3Kinase p85 beta, PIK3R2

Entrez Gene IDs

5296 (Human)

Gene Symbol

PIK3R2

Additional PI 3-Kinase p85 beta Products

Product Documents for PI 3-Kinase p85 beta Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for PI 3-Kinase p85 beta Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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