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PIAS2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-52022

Novus Biologicals, part of Bio-Techne
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NBP1-52022

Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Bovine (100%), Canine (100%), Mouse (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

Product Specifications

Immunogen

Peptide with sequence PQQVREICISRD corresponding to internal region according to NP_004662.2.

Epitope

PQQVREICISRD

Specificity

This antibody is expected to recognize both reported isoforms (NP_775298.1; NP_004662.2).

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for PIAS2 Antibody

Western Blot: PIAS2 Antibody [NBP1-52022]

Western Blot: PIAS2 Antibody [NBP1-52022]

Western Blot: PIAS2 Antibody [NBP1-52022] - WB analysis of PIAS2 using anti-PIAS2 antibody on Jurkat cell lyste (30 ug cell lysate boiled in lysis buffer). Image from verified customer review.
Western Blot: PIAS2 Antibody [NBP1-52022]

Western Blot: PIAS2 Antibody [NBP1-52022]

Western Blot: PIAS2 Antibody [NBP1-52022] - Western blot analysis of PIAS2 (aa185-196) in Human Peripheral Lymphocytes lysate (35ug protein in RIPA buffer) using NBP1-52022 at 1ug/ml. Primary incubation was 1 hour. Detected by chemiluminescence.

Applications for PIAS2 Antibody

Application
Recommended Usage

Peptide ELISA

Detection limit 1:16000

Western Blot

1 - 3 ug/ml
Application Notes
WB: Approx. 60 kDa band observed in human spleen and peripheral mononucleocytes lysates (calculated MW of 63.4 kDa band according to NP_775298.1).
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 4 using NBP1-52022 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: PIAS2

This gene encodes a protein involved in the regulation of transcription factors involved in MAP kinase signaling. The symbol MIZ1 has also been associated with ZBTB17 which is a different gene located on chromosome 1. Two alternatively spliced transcripts encoding different isoforms have been described.

Long Name

Protein Inhibitor of Activated STAT 2

Alternate Names

ARIP3, DIP, MIZ1, PIASx alpha, SIZ2, ZMIZ4

Entrez Gene IDs

9063 (Human); 17344 (Mouse); 83422 (Rat)

Gene Symbol

PIAS2

Additional PIAS2 Products

Product Documents for PIAS2 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for PIAS2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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