Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Human

Applications

Validated:

Immunoprecipitation, Western Blot

Cited:

Chromatin Immunoprecipitation (ChIP), Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1.0 mg/ml

View all PIBF1 Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 685 and 735 of human progesterone-induced-blocking factor 1 using the numbering given in entry NP_006337.2 (GeneID 10464).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PIBF1 Antibody - BSA Free

Western Blot: PIBF1 Antibody [NB100-58834]

Western Blot: PIBF1 Antibody [NB100-58834]

Western Blot: PIBF1 Antibody [NB100-58834] - Detection of Human PIBF1 by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-PIBF1 antibody NB100-58834 used for IP at 3 mcg/mg lysate. PIBF1 was also immunoprecipitated by rabbit anti-PIBF1 antibody NB100-58835, which recognizes a downstream epitope. Detection: Chemiluminescence with an exposure time of 10 seconds.

Applications for PIBF1 Antibody - BSA Free

Application
Recommended Usage

Immunoprecipitation

1:10-1:500

Western Blot

1:100-1:2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: PIBF1

PIBF (progesterone-induced blocking factor 1) is synthesized during pregnancy in response to progesterone by progesterone receptor-positive T lymphocytes (mostly gamma-delta T cells). In the presence of PIBF, natural killer (NK) cells inhibit the release of perforin from storage granules and, therefore, fail to lyse target cells. In humans, the amount of cells that express PIBF is significantly higher in healthy pregnant women than in women at risk for premature pregnancy termination. PIBF has a predicted 89 kDa molecular mass; the full-length PIBF is associated with the nucleus, whereas secretion of shorter forms, such a 34 kDa protein is induced by activation of the cell. Research suggests that PIBF functions as a transcription factor in its full-length form, while smaller forms may act as cytokines.

Long Name

Progesterone Immunomodulatory Binding Factor 1

Alternate Names

C13orf24, chromosome 13 open reading frame 24, KIAA1008, PIBF, progesterone immunomodulatory binding factor 1, progesterone-induced blocking factor 1, progesterone-induced-blocking factor 1, RP11-505F3.1

Entrez Gene IDs

10464 (Human)

Gene Symbol

PIBF1

UniProt

NP_006337.2 (Human)

Additional PIBF1 Products

Product Documents for PIBF1 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for PIBF1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.