PICALM Antibody (PSH01-63)

Western Blot: PICALM Antibody (PSH01-63) [NBP3-32821]

Western Blot: PICALM Antibody (PSH01-63) [NBP3-32821] - Western blot analysis of PICALM on different lysates with Rabbit anti-PICALM antibody (NBP3-32821) at 1/1,000 dilution.

Lane 1: HepG2 cell lysate
Lane 2: PC-3M cell lysate
Lane 3: A431 cell lysate
Lane 4: K-562 cell lysate
Lane 5: Neuro-2a cell lysate
Lane 6: NIH/3T3 cell lysate
Lane 7: PC-12 cell lysate

Lysates/proteins at 20 ug/Lane.

Predicted band size: 71 kDa
Observed band size: 65/71 kDa

Exposure time: 3 minutes;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32821) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.

Immunohistochemistry: PICALM Antibody (PSH01-63) [NBP3-32821]

Immunohistochemistry: PICALM Antibody (PSH01-63) [NBP3-32821] - Immunohistochemical analysis of paraffin-embedded human breast tissue with Rabbit anti-PICALM antibody (NBP3-32821) at 1/50 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32821) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.